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| Status |
Public on Dec 10, 2019 |
| Title |
RNA sequencing analysis of hepatic transcriptome profiles in experimental nonalcoholic steatohepatitis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Two-month-old C57BL/6J male mice were placed on either chow diet or a diet enriched in high fat, cholesterol, and fructose (Research diet D09100301: 40 kcal% fat, 2% cholesterol, 20 kcal% fructose, HFCF diet) for 1 or 3 months. RNA-seq was used to analyze hepatic gene expression from mice on 1-month chow diet, 1-month HFCF diet, 3-month chow diet, and 3-month HFCF.
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| Overall design |
Total RNA for Illumina sequencing was extracted from mouse liver tissues using a Direct-zol RNA kit. During RNA isolation, DNA was removed by treating the samples with RNase-free DNase to avoid DNA contamination. Three biological replicates in each group for a total of 12 samples were submitted for RNA sequencing.
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| Contributor(s) |
Magee NS, Zhang Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 29, 2019 |
| Last update date |
Dec 10, 2019 |
| Contact name |
Yuxia Zhang |
| E-mail(s) |
lzhang5@kumc.edu
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| Phone |
9135881749
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| Organization name |
University of Kansas Medical Center
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| Department |
Pharmacology, Toxicology, and Therapeutics
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| Street address |
3901 Rainbow Blvd, Hemenway 4001
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| City |
kansas city |
| State/province |
KS |
| ZIP/Postal code |
66160 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA557225 |
| SRA |
SRP216746 |
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