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| Status |
Public on Sep 09, 2019 |
| Title |
BRG1 attenuates colonic inflammation and tumorigenesis through autophagy-dependent oxidative stress sequestration |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report that BRG1, an ATPase subunit of the SWI/SNF chromatin remodeling complex, was required for the homeostatic maintenance of colonic epithelial cells (IECs) to prevent the inflammation and tumorigenesis. Consistent with the reduced BRG1 expression in IBD patients, adult mice with IEC ablation of BRG1 developed spontaneous colitis and exhibited increased susceptibility to mutagen-induced tumor growth. Conversely, BRG1 overexpression protected the mice from DSS-induced epithelial damage and subsequent oncogenesis. Mechanistically, BRG1 emerged as a key regulator that directly governs the transcription of Atg16l1, Ambra1, Atg7 and Wipi2, which are important for autophagosome biogenesis. Thus, defective autophagy in BRG1-deficient IECs resulted in excess reactive oxygen species (ROS), which led to the defects in cellular apoptosis and barrier integrity.
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| Overall design |
ChIP assays of two-month-old Brg1F/F and Brg1IEC-AKO IECs were generated by deep sequencing
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| Contributor(s) |
Liu M, Sun T |
| Citation(s) |
31601814 |
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| Submission date |
Jul 29, 2019 |
| Last update date |
Oct 21, 2019 |
| Contact name |
min liu |
| E-mail(s) |
liumin993228@sjtu.edu.cn
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| Phone |
13681629552
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| Organization name |
shanghaijiaotong university
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| Street address |
huashan road
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| City |
shanghai |
| ZIP/Postal code |
200030 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA557213 |
| SRA |
SRP216708 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE135041_RAW.tar |
7.6 Mb |
(http)(custom) |
TAR (of XLSX) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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