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| Status |
Public on Jul 26, 2019 |
| Title |
RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of H1, H1 derived APLNR+ cells, CD31+CD34+ cells and CD43+ cells during human early hematopoietic differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The goals of this study are to verify the dynamic changes of MAGs in H1 derived different population of cells during human early hematopoietic differentian.
Methods: mRNA profiles of hESC samples collected from day 0 to day 8 after hematopoiesis differentiation were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays
Conclusions:MAGs showed convincingly dynamic expression during early hematopoietic differentiation of H1 cells
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| Overall design |
Transcriptomes of H1, H1 derived APLNR+ cells, CD31+CD34+ cells and CD43+ cells during human early hematopoietic differentiation
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| Contributor(s) |
Zhou J, Wang H |
| Citation(s) |
33101849, 33319461 |
| |
| Submission date |
Jul 25, 2019 |
| Last update date |
Jan 19, 2021 |
| Contact name |
Jiaxi Zhou |
| E-mail(s) |
leisheng_zhang@163.com
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| Phone |
+86-22-23909101
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| Organization name |
PUMC
|
| Street address |
288# Nanjing Road, He ping district
|
| City |
Tianjin |
| State/province |
Tianjin |
| ZIP/Postal code |
300020 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA556721 |
| SRA |
SRP216438 |
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