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Series GSE134705 Query DataSets for GSE134705
Status Public on Dec 12, 2019
Title Cross-species analysis across 450 million years of evolution reveals conservation and divergence of the microglia program (scRNA-seq)
Organisms Danio rerio; Gallus gallus; Callithrix jacchus; Macaca fascicularis; Homo sapiens; Ovis aries; Mesocricetus auratus; Mus musculus; Rattus norvegicus; Nannospalax galili
Experiment type Expression profiling by high throughput sequencing
Summary Here we characterize microglia transcriptional program across ten species spanning more than 450 million years of evolution. We find that microglia express a conserved core gene program of orthologous genes from rodents to human, including ligands and receptors associated with interactions between glia and neurons. In most species, microglia show a single dominant transcriptional state, while humans express significant microglia heterogeneity. In addition, we observed notable differences in complement, phagocytic and several critical signaling pathways that are enriched with susceptibility genes to brain disorders, including Alzheimer’s and Parkinson’s disease in microglia of common animal models as compared to human. Our study provides an essential resource of conserved and divergent microglia pathways across evolution with important implications for future development of microglia-based therapies in humans.
Overall design Single cell and bulk RNA-seq of microglia from 10 different species and comparison of transcriptional landcape over species. Biological replicates of different species were n=3-6.
Contributor(s) Geirsdottir L, Amit I, David E, Weiner A, Keren-Shaul H, Ulitsky I
Citation(s) 31835035
Submission date Jul 23, 2019
Last update date Jun 17, 2020
Contact name Ido Amit
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
Platforms (10)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL19787 Illumina NextSeq 500 (Gallus gallus)
Samples (37)
GSM3963869 AB3501
GSM3963870 AB3018
GSM3963871 AB5662
This SubSeries is part of SuperSeries:
GSE134707 Cross-species analysis across 450 million years of evolution reveals conservation and divergence of the microglia program
BioProject PRJNA556200
SRA SRP216250

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134705_RAW.tar 16.8 Mb (http)(custom) TAR (of TXT)
GSE134705_metadata_t.txt.gz 283.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file

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