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| Status |
Public on Nov 05, 2008 |
| Title |
Effect of wt versus mutant hsa-miR-122 overexpression on spontaneous hESC differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We aimed to determine whether overexpression of endoderm-specific miRNA may affect hESC differentiation. To this end, we analyzed the effect of lentiviral-based overexpression of liver-specific miR-122 on hESC differentiation, using genomewide gene microarrays. Stable overexpression of endoderm-specific miR-122 in hESC resulted in increased expression of a few endodermal markers in spontaneously-differentiating hESC, but had no clear effect on directing differentiation towards an endodermal fate; rather, it delayed the general differentiation of hESC.
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| Overall design |
Lentiviral vectors encoding either wt or mutant (control) hsa-miR-122 were transduced into hES cells (line HES2). The cells differentiated spontaneously in KO medium without feeder cells for 2 weeks, and the gene expression profile of wt- vs mutant-expressing miR-122 was compared. We used for this study 3 arrays for control (mutant miR) and 2 arrays for the treatment (wt miRNA).
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| Contributor(s) |
Tzur G, Levy A, Meiri E, Barad O, Spector Y, Bentwich Z, Mizrahi L, Katzenellenbogen M, Ben-Shushan E, Reubinoff B, Galun E |
| Citation(s) |
19015728 |
| |
| Submission date |
Nov 04, 2008 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Galit Tzur |
| E-mail(s) |
galit.tzurlevy@mail.huji.ac.il
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| Phone |
972-2-6778948
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| Fax |
972-2-6430982
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| Organization name |
Hadassah University Hospital
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| Department |
Gene Therapy
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| Lab |
Eithan Galun
|
| Street address |
Kiryat Hadassah
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| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA110059 |
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