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Series GSE13460 Query DataSets for GSE13460
Status Public on Nov 05, 2008
Title Effect of wt versus mutant hsa-miR-122 overexpression on spontaneous hESC differentiation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We aimed to determine whether overexpression of endoderm-specific miRNA may affect hESC differentiation. To this end, we analyzed the effect of lentiviral-based overexpression of liver-specific miR-122 on hESC differentiation, using genomewide gene microarrays. Stable overexpression of endoderm-specific miR-122 in hESC resulted in increased expression of a few endodermal markers in spontaneously-differentiating hESC, but had no clear effect on directing differentiation towards an endodermal fate; rather, it delayed the general differentiation of hESC.
Overall design Lentiviral vectors encoding either wt or mutant (control) hsa-miR-122 were transduced into hES cells (line HES2). The cells differentiated spontaneously in KO medium without feeder cells for 2 weeks, and the gene expression profile of wt- vs mutant-expressing miR-122 was compared. We used for this study 3 arrays for control (mutant miR) and 2 arrays for the treatment (wt miRNA).
Contributor(s) Tzur G, Levy A, Meiri E, Barad O, Spector Y, Bentwich Z, Mizrahi L, Katzenellenbogen M, Ben-Shushan E, Reubinoff B, Galun E
Citation(s) 19015728
Submission date Nov 04, 2008
Last update date Dec 06, 2018
Contact name Galit Tzur
Phone 972-2-6778948
Fax 972-2-6430982
Organization name Hadassah University Hospital
Department Gene Therapy
Lab Eithan Galun
Street address Kiryat Hadassah
City Jerusalem
ZIP/Postal code 91120
Country Israel
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (5)
GSM339011 HES2_Mir122Mut_rep1
GSM339012 HES2_Mir122WT_rep1
GSM339425 HES2_Mir122Mut_rep2
BioProject PRJNA110059

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Supplementary file Size Download File type/resource
GSE13460_RAW.tar 9.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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