Expression profiling by high throughput sequencing
Summary
We molecularly characterized thousands of intestinal cells including epithelial, masenchymal and immune cells using scRNAseq. This enable us to uncover the important role of villus tip telocytes as master regulators of the epithelial spatial expression programs along the villus axis
Overall design
mRNA profiles from single cells, EDTA isolated jejunum epithelial cells and LCM samples extracted from mouse jejunum were sequenced in several batches in an Illumina NextSeq.