|
| Status |
Public on Jun 03, 2020 |
| Title |
Mapping Gata2 enhancer-dependent developmental trajectories by single-cell RNA-sequencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We compared single-cell transcriptomes of fetal liver myeloid progenitor pools (CMP/GMP pool) from Gata2 -77 enhancer mutant embryos and wildtype littermates. Gene expression patterns in -77-deficient mice demonstrated loss of erythroid, megakaryocyte and granulocyte progenitors and an increase in monocyte
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| |
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| Overall design |
Lineage-Sca1-cKit+CD34+ cells from E14.5 fetal livers of two wild type and two -77-deleted embryos were processed for single-cell RNA sequening using 10x Genomics Chromium platform.
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| Contributor(s) |
Bresnick EH, Keles S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 17, 2019 |
| Last update date |
Jun 03, 2020 |
| Contact name |
Emery Bresnick |
| E-mail(s) |
dconn2@wisc.edu
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Cell and Regenerative Biology
|
| Lab |
Bresnick Lab
|
| Street address |
8457 WIMR 1111 Highland Ave.
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA555423 |
| SRA |
SRP215528 |
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