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| Status |
Public on Dec 14, 2020 |
| Title |
Transcriptome analysis of naive B cells from CD19Cre and CD19Cre_STAT6vt mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate how activated STAT6 modulates B cells development and function in vivo, we characterized mice that express a constitutively active version specifically in B cells. CD19Cre_STAT6vt mice show spontaneous phosphorylation and nuclear translocation of STAT6 in B cells. Based on the finding that STAT6vt accumulates in the nucleus of naïve splenic B cells in CD19Cre_STAT6vt mice, we next determined the changes of the global transcriptome by RNAseq analysis.
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| Overall design |
We isolated naïve B cells by cell sorting (CD19+ CD3neg PIneg, purity >95%) from three age matched CD19Cre_STAT6vt and three CD19Cre control mice, purified total RNA and performed transcriptome analysis using the Illumina platform.
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| Contributor(s) |
Haase P, Radkte D, Voehringer D |
| Citation(s) |
31803941 |
| |
| Submission date |
Jul 17, 2019 |
| Last update date |
Jan 28, 2021 |
| Contact name |
David Voehringer |
| E-mail(s) |
david.voehringer@uk-erlangen.de
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| Organization name |
University Clinic Erlangen
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| Department |
Department of Infection Biology
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| Street address |
Wasserturmstrasse 3-5
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| City |
Erlangen |
| ZIP/Postal code |
91054 |
| Country |
Germany |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA555114 |
| SRA |
SRP215247 |
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