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| Status |
Public on Sep 02, 2021 |
| Title |
Defining the genetic and immune landscape of precursor myeloma progression with single-cell sequencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Multiple Myeloma is universally preceded by a pre-malignant state, however, the genetic and immune mechanisms associated with transformation are incompletely understood thus hindering development of preventative treatment strategies. Using single-cell RNA sequencing, we profiled the transcriptomes of tumor and immune microenvironment cells derived from transgenic mice whose disease spanned the myeloma progression spectrum. This work in turn provides unique and previously unappreciated insight into the biology of precursor disease progression.
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| Overall design |
BM were extracted from hind-leg bones of VkMYC/non-transgenic control mice, dissociated into a single cell suspension using mechanical disruption (syringe) and RBC's lysed. Single-cell RNA-sequencing libraries were then generated using the 10x Genomics Chromium Single Cell 3' Solution V2 protocol
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| Contributor(s) |
Croucher D, Pugh TJ |
| Citation(s) |
34732728 |
| |
| Submission date |
Jul 16, 2019 |
| Last update date |
Dec 02, 2021 |
| Contact name |
Danielle Croucher |
| E-mail(s) |
d.croucher@mail.utoronto.ca
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| Organization name |
University of Toronto
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| Department |
Medical Biophysics
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| Street address |
101 College Street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA554883 |
| SRA |
SRP214856 |
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