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| Status |
Public on Jan 11, 2021 |
| Title |
Protein-coding and non-coding RNA expression in whole blood leukocytes during experimental human endotoxemia. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Genome-wide gene expression profiling of whole blood leukocytes during experimental human endotoxemia has been used extensively to model acute systemic host responses. Through technological advances in genomics it has become clear that transcription is not limited to protein-coding regions of the genome. Here, we describe a comprehensive analysis of RNA expression of blood leukocytes in healthy volunteers during experimental human endotoxemia.
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| Overall design |
Whole-blood transcriptional patterns of protein-coding and non-coding RNA molecules were generated by means of the human transcriptome array 2.0 (HTA 2.0) chip. Whole blood of healthy male participants was collected before and 2, 4, 6 and 24 hours after intravenous administration of 2ng/kg Escherichia coli lipopolysaccharide (LPS). The Dutch Central Committee on Research Involving Human Subjects (CCMO) and the Medical Ethics Committee of the Academic Medical Center (AMC), Amsterdam, the Netherlands, approved the study. Title legend: endo_0h, healthy subjects before LPS administration; endo_2h, endo_4h, endo_6h, endo_24h, healthy subjects after 2, 4, 6 and 24 hours LPS administration, respectively; endo_dup_0h, duplicate samples of healthy subjects before LPS administration.
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| Contributor(s) |
Scicluna BP, van der Poll T |
| Citation(s) |
33305733 |
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| Submission date |
Jul 16, 2019 |
| Last update date |
Jan 11, 2021 |
| Contact name |
Brendon Scicluna |
| E-mail(s) |
brendon.scicluna@um.edu.mt
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| Organization name |
University of Malta
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| Street address |
Msida campus
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| City |
Msida |
| ZIP/Postal code |
MSD2020 |
| Country |
Malta |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE134364 |
Protein-coding and non-coding RNA landscape in critically ill patients with sepsis |
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| Relations |
| BioProject |
PRJNA554855 |
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