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Status |
Public on Jun 01, 2020 |
Title |
Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells (Mnase-seq & ChIP-seq) |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We employed an MNase-Transcription Start Site Sequence Capture method to map and determine the accessibility of all nucleosomes, including those that contain H2A.Z, at high coverage for all human Pol II promoters. We uncovered unexpected features of nucleosomal organization in epithelial cells and following transition to a mesenchymal and malignant state. In contrast to the prevailing model, we observe many different types of active and inactive promoter structures that differ in their nucleosome organization and sensitivity to MNase digestion. Further, we found that H2A.Z has an important role in the assembly of repressed promoters into an inaccessible state. Finally, we uncovered a new promoter type in which a stable H2A.Z nucleosome occupies the TSS of an active promoter.
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Overall design |
Nucleosome mapping via Mnase-seq and parallel ChIP-seq of H2A.Z in MCF10A and MCF10A-CA1a cells
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Contributor(s) |
Dennis J, Cole L, Kurscheid S, Tremethick D |
Citation(s) |
33953180 |
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Submission date |
Jul 15, 2019 |
Last update date |
Nov 07, 2023 |
Contact name |
LAUREN A COLE |
E-mail(s) |
LC13G@MY.FSU.EDU
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Phone |
8506458573
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Organization name |
FLORIDA STATE UNIVERSITY
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Street address |
319 STADIUM DR
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City |
TALLAHASSEE |
State/province |
Florida |
ZIP/Postal code |
32304 |
Country |
USA |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (28)
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This SubSeries is part of SuperSeries: |
GSE134299 |
Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells |
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Relations |
BioProject |
PRJNA554664 |
SRA |
SRP214664 |
Supplementary file |
Size |
Download |
File type/resource |
GSE134297_combined_H_A_H2AZ_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
6.2 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_H_A_Inp_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
6.1 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_H_CA1a_H2AZ_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
8.1 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_H_CA1a_Inp_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
5.4 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_H_TGFb_H2AZ_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
6.7 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_H_TGFb_Inp_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
6.1 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_H_shH2AZ_Inp_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
5.9 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_L_A_H2AZ_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
4.1 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_L_A_Inp_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
4.3 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_L_CA1a_H2AZ_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
4.1 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_L_CA1a_Inp_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
4.6 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_L_TGFb_H2AZ_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
3.6 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_L_TGFb_Inp_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
3.8 Mb |
(ftp)(http) |
TXT |
GSE134297_combined_L_shH2AZ_Inp_centers_seqcap_targets_w60s10_scg3_copy.mat10.txt.gz |
5.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |