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| Status |
Public on Feb 02, 2009 |
| Title |
High-throughput pyrosequencing of endogenous small RNAs from Arabidopsis thaliana wild-type and ddm1 mutant. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
DNA methylation is essential for silencing transposable elements and some genes in higher eukaryotes, implying that this modification must be tightly controlled. However, accidental changes in DNA methylation can be transmitted through mitosis, as in cancer, or meiosis, leading to epiallelic variation. Here, we demonstrate the existence of an efficient and faithful mechanism that protects against transgenerational loss of DNA methylation in the plant Arabidopsis. This process is specific to the subset of heavily methylated genomic repeats that are targeted by the RNAi machinery, and does not spread into flanking regions. Remethylation is often progressive over two to four sexual generations. This differential and incremental correction of epigenetic defects may preserve genome stability while increasing adaptive opportunities.
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| Overall design |
2 samples examined: wild type, and ddm1 mutant.
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| Contributor(s) |
Teixeira FK, Heredia F, Sarazin A, Roudier F, Boccara M, Ciaudo C, Cruaud C, Poulain J, Berdasco M, Fraga MF, Voinnet O, Wincker P, Esteller M, Colot V |
| Citation(s) |
19179494 |
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| Submission date |
Oct 31, 2008 |
| Last update date |
May 15, 2019 |
| Contact name |
Alexis Sarazin |
| E-mail(s) |
sarazin@biologie.ens.fr
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| Phone |
+33 1 44 32 35 24
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| Organization name |
CNRS UMR 8186 - Biologie Moléculaire des Organismes Photosynthétiques
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| Department |
Ecole Normale Supérieure, Département de Biologie
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| Lab |
Arabidopsis Epigenetics and Epigenomics
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| Street address |
46 rue d'Ulm
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| City |
PARIS |
| ZIP/Postal code |
75230 |
| Country |
France |
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| Platforms (1) |
| GPL9386 |
454 GS (Arabidopsis thaliana) |
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| Samples (2) |
| GSM338557 |
Endogenous small RNAs from Arabidopsis thaliana col-0, wild type, seedlings |
| GSM338558 |
Endogenous small RNAs from Arabidopsis thaliana col-0, ddm1 mutant, seedlings |
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| Relations |
| SRA |
SRP001257 |
| BioProject |
PRJNA110069 |
| Supplementary data files not provided |
SRA Run Selector |
| Processed data included within Sample table |
| Raw data are available in SRA |
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