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Series GSE134144 Query DataSets for GSE134144
Status Public on Jan 13, 2020
Title The Dynamic Transcriptional Cell Atlas of Testis Development During Human Puberty
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The human testis undergoes dramatic developmental and structural changes during puberty, including proliferation and maturation of niche/somatic cells, and the onset of spermatogenesis. Here, we profiled and analyzed single-cell transcriptomes of ~10,000 testicular cells from four boys spanning puberty, and compared to infants and adults. During puberty, undifferentiated spermatogonia first expand and then differentiate, prior to gametogenesis. Notably, we identify a common pre-pubertal progenitor for Leydig and myoid cells, and reveal candidate factors/pathways for pubertal differentiation. Furthermore, pre-pubertal Sertoli cells form two states that differ in mitochondrial/metabolic transcription, which converge to a single mature population during puberty. Roles for testosterone in Sertoli cell maturation, antimicrobial peptide secretion and spermatogonial differentiation are revealed through analyses of testosterone-suppressed transgender female testis and via in vitro seminiferous tubule culturing. Overall, our transcriptional atlas of the developing human testis provides major insights into developmental changes and key factors/pathways that accompany male puberty.
Overall design Single-cell RNA-seq of testicular cells from different ages of boys in puberty. Testosterone-suppressed transgender female testis samples are included.
Contributor(s) Guo J, Cairns B
Citation(s) 31928944
Submission date Jul 11, 2019
Last update date Feb 01, 2020
Contact name Jingtao Guo
Phone 801-585-1823
Organization name University of Utah School of Medicine
Department Department of Surgery Division of Urology
Lab Jingtao Guo
Street address 2000 Circle of Hope
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (11)
GSM3937918 7yo_rep1
GSM3937919 7yo_rep2
GSM3937920 11yo_rep1
BioProject PRJNA554092
SRA SRP214255

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134144_Pubertal_combined_UMI.txt.gz 28.1 Mb (ftp)(http) TXT
GSE134144_Transgender_combined_UMI.txt.gz 14.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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