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| Status |
Public on Aug 15, 2019 |
| Title |
Serum-free media supplements carry miRNAs that co-purify with extracellular vesicles (EVs) |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
With the goal to identify miRNAs and other small RNAs involved in EV-mediated oligodendrocyte-neuron communication, we performed deep sequencing (RNA-seq) of small RNAs derived from EVs isolated from culture supernatants of primary mouse oligodendrocytes by differential centrifugation. Oligodendrocytes were cultured and EVs harvested under serum-free conditions in presence of B27 supplement. We found that RNA isolated from EVs harvested from cells under serum-replacement conditions contains miRNA contaminants carried into the sample by defined media components.
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| |
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| Overall design |
Small RNA expression profiling of extracellular vesicles (EVs) derived from mouse oligodendrocytes
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| |
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| Contributor(s) |
Auber M, Fröhlich D, Drechsel O, Karaulanov E, Krämer-Albers E |
| Citation(s) |
31552133 |
| |
| Submission date |
Jul 10, 2019 |
| Last update date |
Sep 26, 2019 |
| Contact name |
Emil Karaulanov |
| Organization name |
Institute of Molecular Biology
|
| Lab |
Bioinformatics Core Facility
|
| Street address |
Ackermannweg 4
|
| City |
Mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA553766 |
| SRA |
SRP214112 |
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