|Public on Oct 01, 2009
|Effect of T0901317, AM580 or T0901317+AM580 on monocyte gene expression
|Expression profiling by array
|The aim of this study was to investigate the effect of T0901317 LXR agonist on Retinoic Acid Receptor (RAR) family gene expression. T0901317 alone increase RARalpha gene expression in monocytes and the effects of the co treatment of the cells with T0901317 and a specific RARalpha agonist (AM580) were further studied. For this monocytes were treated for 24h with DMSO or T0901317 to induce RARalpha expression and in a second step cells were treated or not with AM580 for another 24h period. Gene expression was analysed for all the treatments (T0901317, AM580 or T0901317+AM580) and we focussed on genes which expression was synergitically induced by the co treatment as compared to the cells treated either by T0901317 or AM580.
|Monocytes were obtained from 2 healthy donors with inform consent. Cells were treated in vitro with the vehicle (DMSO 0,1%) or 10µM of the LXR agonist T0901317 for 24 hours. Then after cells were treated for another 24 hour period with or without 100nM of the RARalpha specific agonist AM580.
|Rebe C, Raveneau M, Chevriaux A, Lakomy D, Costa A, Bessede G, Solary E, Gambert P, Masson D
|Oct 30, 2008
|Last update date
|Oct 29, 2019
|Hopital du Bocage
|2 av marechal de Lattre de Tassigny
|Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
|Monocytes_100 nM AM580 48h_donor1
|Monocytes_10 µM T0901317 48h_donor1
|Monocytes_10µM T0901317 + 100nM AM580 48h_donor1