|
| Status |
Public on May 28, 2020 |
| Title |
UPF1 regulatory loop variant RNA-Seq and RIP-Seq |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
HEK-293 Trex cells containing stable tetracycline-inducible transgenes driving overexpression of CLIP-tagged UPF1 isoforms 1 and 2 or GFP were used for total RNA-seq. In addition, CLIP-UPF1 isoforms were affinity purified, and bound RNA was subjected to high-throughput sequencing.
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| |
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| Overall design |
Paired-end high-throughput RNA sequencing of Total RNA from cells expressing CLIP-tagged UPF1 isoform 1, UPF1 isoform 2, or GFP and RNA associated with affinity-purified CLIP UPF1 isoforms 1 and 2.
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| |
|
| Contributor(s) |
Fritz SE, Hogg JR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 09, 2019 |
| Last update date |
May 28, 2020 |
| Contact name |
J. Robert Hogg |
| E-mail(s) |
j.hogg@nih.gov
|
| Organization name |
National Heart, Lung, and Blood Institute
|
| Department |
Biochemistry and Biophysics Center
|
| Street address |
50 South Drive, Room 2341
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (15)
|
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| Relations |
| BioProject |
PRJNA553576 |
| SRA |
SRP213944 |
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