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Series GSE134059 Query DataSets for GSE134059
Status Public on May 28, 2020
Title UPF1 regulatory loop variant RNA-Seq and RIP-Seq
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary HEK-293 Trex cells containing stable tetracycline-inducible transgenes driving overexpression of CLIP-tagged UPF1 isoforms 1 and 2 or GFP were used for total RNA-seq. In addition, CLIP-UPF1 isoforms were affinity purified, and bound RNA was subjected to high-throughput sequencing.
Overall design Paired-end high-throughput RNA sequencing of Total RNA from cells expressing CLIP-tagged UPF1 isoform 1, UPF1 isoform 2, or GFP and RNA associated with affinity-purified CLIP UPF1 isoforms 1 and 2.
Contributor(s) Fritz SE, Hogg JR
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Submission date Jul 09, 2019
Last update date May 28, 2020
Contact name J. Robert Hogg
Organization name National Heart, Lung, and Blood Institute
Department Biochemistry and Biophysics Center
Street address 50 South Drive, Room 2341
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (15)
GSM3935778 UPF1_isoform_1_Total_1
GSM3935779 UPF1_isoform_1_Total_2
GSM3935780 UPF1_isoform_1_Total_3
BioProject PRJNA553576
SRA SRP213944

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Supplementary file Size Download File type/resource
GSE134059_Processed_data.txt.gz 3.5 Mb (ftp)(http) TXT
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Processed data are available on Series record

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