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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 21, 2019 |
| Title |
TREM2 regulates microglial lipid metabolism during aging in mice [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
TREM2 is a microglial-specific gene implicated in late-onset Alzheimer's Disease (AD). Recent studies have identified that Trem2-deficient mice exhibit transcriptional changes in microglial gene expression that minimize the upregulation of lipid metabolism and lysosomal genes in AD disease models. We detect similiarly attenuated expression of lipid metabolism genes in microglia isolated from brains of aged Trem2 knockout mice.
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| Overall design |
Microglia and astrocytes were purified from brains of Trem2 wildtype and homozygous knockout mice of young (2 months) and old (16-18 months) mice using fluorescent activated cell sorting (FACS). Gene expression was assessed using 3' tag sequencing of poly-adenylated RNA (QuantSeq, Lexogen, Vienna, Austria) with unique-molecular identifiers (UMIs).
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| Contributor(s) |
Nugent AA, Sandmann T, Monroe KM, Di Paolo G |
| Citation(s) |
31902528 |
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| Submission date |
Jul 09, 2019 |
| Last update date |
Mar 22, 2020 |
| Contact name |
Thomas Sandmann |
| E-mail(s) |
genomics@dnli.com, sandmann@dnli.com
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| Organization name |
Denali Therapeutics
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| Street address |
161 Oyster Point Blvd
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| City |
South San Francisco |
| State/province |
California |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (56)
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| GSM3933552 |
microglia, TREM2 -/-, young IL10_M |
| GSM3933553 |
astrocyte, TREM2 +/+, old IL11_A |
| GSM3933554 |
microglia, TREM2 +/+, old IL11_M |
| GSM3933555 |
astrocyte, TREM2 -/-, old IL12_A |
| GSM3933556 |
microglia, TREM2 -/-, old IL12_M |
| GSM3933557 |
astrocyte, TREM2 +/+, young IL13_A |
| GSM3933558 |
microglia, TREM2 +/+, young IL13_M |
| GSM3933559 |
astrocyte, TREM2 -/-, young IL14_A |
| GSM3933560 |
microglia, TREM2 -/-, young IL14_M |
| GSM3933561 |
astrocyte, TREM2 +/+, old IL15_A |
| GSM3933562 |
microglia, TREM2 +/+, old IL15_M |
| GSM3933563 |
astrocyte, TREM2 -/-, old IL16_A |
| GSM3933564 |
microglia, TREM2 -/-, old IL16_M |
| GSM3933565 |
astrocyte, TREM2 +/+, young IL17_A |
| GSM3933566 |
microglia, TREM2 +/+, young IL17_M |
| GSM3933567 |
astrocyte, TREM2 -/-, young IL18_A |
| GSM3933568 |
microglia, TREM2 -/-, young IL18_M |
| GSM3933569 |
astrocyte, TREM2 +/+, old IL19_A |
| GSM3933570 |
microglia, TREM2 +/+, old IL19_M |
| GSM3933571 |
astrocyte, TREM2 -/-, young IL2_A |
| GSM3933572 |
microglia, TREM2 -/-, young IL2_M |
| GSM3933573 |
astrocyte, TREM2 -/-, old IL20_A |
| GSM3933574 |
microglia, TREM2 -/-, old IL20_M |
| GSM3933575 |
astrocyte, TREM2 +/+, young IL21_A |
| GSM3933576 |
microglia, TREM2 +/+, young IL21_M |
| GSM3933577 |
astrocyte, TREM2 -/-, young IL22_A |
| GSM3933578 |
microglia, TREM2 -/-, young IL22_M |
| GSM3933579 |
astrocyte, TREM2 +/+, old IL23_A |
| GSM3933580 |
microglia, TREM2 +/+, old IL23_M |
| GSM3933581 |
astrocyte, TREM2 -/-, old IL24_A |
| GSM3933582 |
microglia, TREM2 -/-, old IL24_M |
| GSM3933583 |
astrocyte, TREM2 +/+, young IL25_A |
| GSM3933584 |
microglia, TREM2 +/+, young IL25_M |
| GSM3933585 |
astrocyte, TREM2 -/-, young IL26_A |
| GSM3933586 |
microglia, TREM2 -/-, young IL26_M |
| GSM3933587 |
astrocyte, TREM2 +/+, old IL27_A |
| GSM3933588 |
microglia, TREM2 +/+, old IL27_M |
| GSM3933589 |
astrocyte, TREM2 -/-, old IL28_A |
| GSM3933590 |
microglia, TREM2 -/-, old IL28_M |
| GSM3933591 |
astrocyte, TREM2 +/+, old IL3_A |
| GSM3933592 |
microglia, TREM2 +/+, old IL3_M |
| GSM3933593 |
astrocyte, TREM2 -/-, old IL4_A |
| GSM3933594 |
microglia, TREM2 -/-, old IL4_M |
| GSM3933595 |
astrocyte, TREM2 +/+, young IL5_A |
| GSM3933596 |
microglia, TREM2 +/+, young IL5_M |
| GSM3933597 |
astrocyte, TREM2 -/-, young IL6_A |
| GSM3933598 |
microglia, TREM2 -/-, young IL6_M |
| GSM3933599 |
astrocyte, TREM2 +/+, old IL7_A |
| GSM3933600 |
microglia, TREM2 +/+, old IL7_M |
| GSM3933601 |
astrocyte, TREM2 -/-, old IL8_A |
| GSM3933602 |
microglia, TREM2 -/-, old IL8_M |
| GSM3933603 |
astrocyte, TREM2 +/+, young IL9_A |
| GSM3933604 |
microglia, TREM2 +/+, young IL9_M |
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| This SubSeries is part of SuperSeries: |
| GSE130627 |
TREM2 regulates microglial cholesterol metabolism upon chronic phagocytic challenge |
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| Relations |
| BioProject |
PRJNA553523 |
| SRA |
SRP213880 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE134031_DST120.tab.gz |
1.3 Mb |
(ftp)(http) |
TAB |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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