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Status |
Public on Apr 03, 2020 |
Title |
Small RNA sequencing of msh1 memory line and with isogenic Col-0 wild-type control in Arabidopsis |
Organism |
Arabidopsis thaliana |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
Col-0 plants were transformed with MSH1 RNAi construct. Transgene positive plant was self pollinated and transgene was segregated in subsequent generation, and screened for presence of transgene using PCR assay. Of transgene null plants, 20% plants displayed delayed in flowering, smaller in size, and lighter green termed as memory phenotype.
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Overall design |
Two memory plants, four Col-0 wild-type plants were sequenced.
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Contributor(s) |
Yang X, Mackenzie S |
Citation(s) |
32371941 |
Submission date |
Jul 09, 2019 |
Last update date |
Dec 22, 2021 |
Contact name |
Hardik Kundariya |
E-mail(s) |
hsk13@psu.edu
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Organization name |
The Pennsylvania State University
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Street address |
361 N. Frear
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City |
University Park |
State/province |
PA |
ZIP/Postal code |
16801 |
Country |
USA |
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Platforms (1) |
GPL24025 |
BGISEQ-500 (Arabidopsis thaliana) |
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Samples (6)
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Relations |
BioProject |
PRJNA553516 |
SRA |
SRP213878 |