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| Status |
Public on Dec 31, 2019 |
| Title |
Functional specification of CCK+ interneurons by alternative isoforms of Kv4.3 auxiliary subunits |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
CCK-expressing interneurons (CCK+INs) are crucial for controlling hippocampal activity. We found two firing phenotypes of CCK+INs in rat CA3 area; either possessing a previously undetected membrane potential-dependent firing or regular firing phenotype, due to different low-voltage-activated potassium currents. These different excitability properties destine the two types for distinct functions, because the former is essentially silenced during realistic 8-15 Hz oscillations. The general excitability, morphology and gene-profiles of the two types were surprisingly similar. Even the expression of Kv4.3 channels were comparable, despite evidences showing that Kv4.3-mediated currents underlie the distinct firing properties. Instead, the firing phenotypes were correlated with the presence of distinct isoforms of Kv4 auxiliary subunits (KChIP1 vs. KChIP4e and DPP6S). Our results reveal the underlying mechanisms of two previously unknown types of CCK+INs and demonstrate that alternative splicing of few genes, which may be viewed as a minor event in the cells’ whole transcriptome, can underlie distinct cell-type identity.
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| Overall design |
mRNA profiles of 17 CCK+/CNR1+ CA3 interneurons, 8 showing rectifying firing (TOR type), 9 showing regular firing (RS type)
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| Contributor(s) |
Oláh VJ, Lukacsovich D, Winterer J, Lörincz A, Nusser Z, Földy C, Szabadics J |
| Citation(s) |
32490811 |
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| Submission date |
Jul 08, 2019 |
| Last update date |
Jun 08, 2020 |
| Contact name |
Csaba Földy |
| E-mail(s) |
single-cell@hifo.uzh.ch
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| Organization name |
University of Zurich
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| Department |
Brain Research Institute
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| Lab |
Foldy Lab
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| Street address |
Winterthurerstrasse 190
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| City |
Zurich |
| State/province |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA553202 |
| SRA |
SRP213647 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133951_RPKM_gene_counts.tsv.gz |
1.2 Mb |
(ftp)(http) |
TSV |
| GSE133951_TPM_gene_counts.tsv.gz |
1.3 Mb |
(ftp)(http) |
TSV |
| GSE133951_gene_counts.tsv.gz |
343.2 Kb |
(ftp)(http) |
TSV |
| GSE133951_normalized_gene_counts.tsv.gz |
943.0 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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