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| Status |
Public on Jul 10, 2021 |
| Title |
MeRIP sequencing analysis of BJAB cell line infected with EBV |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Purpose: Evaluation of the m6A modification of EBV and BJAB transcripts during EBV infection
Methods: Human B lymphoma cell line BJAB was uninfected or infected with EBV for 24 hours. Total RNA from each sample were extracted. Intact mRNA was isolated from total RNA samples and then chemically fragmented to 100-nucleoside-long fragments. m6A methylated mRNAs were immunoprecipitated with anti-N6-methyadenosine (m6A) antibody (a part of the fragmented mRNAs was kept as input). Both m6A enriched mRNAs and input mRNAs were concentrated for RNA-seq library construction. Sequencing was performed using an Illumina HiSeq 4000.
Results: EBV EBNA2 and BHRF1 transcripts were m6A modified and m6A modification of BJAB transcripts changed during EBV infection.
Conclusions: Our study found that some EBV transcripts were m6A modified during EBV infection and EBV infection changed m6A modification profiles of BJAB transcripts.
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| Overall design |
Three independent biological replicates were plated and uninfected or infected with EBV in parallel.
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| Contributor(s) |
Zheng X, Wang J, Ma J |
| Citation(s) |
33434416 |
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| Submission date |
Jul 08, 2019 |
| Last update date |
Oct 09, 2021 |
| Contact name |
Jian Ma |
| E-mail(s) |
majian@csu.edu.cn
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| Organization name |
Central South University
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| Department |
Cancer Research Institute
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| Street address |
110 Xiangya Road
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| City |
Changsha |
| State/province |
Hunan |
| ZIP/Postal code |
410078 |
| Country |
China |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (12)
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| GSM3930367 |
BJAB cells, Uninfected, biological rep 1, m6A-RIP |
| GSM3930368 |
BJAB cells, Uninfected, biological rep 1, input |
| GSM3930369 |
BJAB cells, Uninfected, biological rep 2, m6A-RIP |
| GSM3930370 |
BJAB cells, Uninfected, biological rep 2, input |
| GSM3930371 |
BJAB cells, Uninfected, biological rep 3, m6A-RIP |
| GSM3930372 |
BJAB cells, Uninfected, biological rep 3, input |
| GSM3930373 |
BJAB cells, Infected with EBV, biological rep 1, m6A-RIP |
| GSM3930374 |
BJAB cells, Infected with EBV, biological rep 1, input |
| GSM3930375 |
BJAB cells, Infected with EBV, biological rep 2, m6A-RIP |
| GSM3930376 |
BJAB cells, Infected with EBV, biological rep 2, input |
| GSM3930377 |
BJAB cells, Infected with EBV, biological rep 3, m6A-RIP |
| GSM3930378 |
BJAB cells, Infected with EBV, biological rep 3, input |
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| Relations |
| BioProject |
PRJNA553159 |
| SRA |
SRP213617 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133936_BJAB-Control-1-peaks.xlsx |
3.9 Mb |
(ftp)(http) |
XLSX |
| GSE133936_BJAB-Control-2-peaks.xlsx |
4.9 Mb |
(ftp)(http) |
XLSX |
| GSE133936_BJAB-Control-3-peaks.xlsx |
4.2 Mb |
(ftp)(http) |
XLSX |
| GSE133936_BJAB-EBV-1-peaks.xlsx |
4.9 Mb |
(ftp)(http) |
XLSX |
| GSE133936_BJAB-EBV-2-peaks.xlsx |
4.7 Mb |
(ftp)(http) |
XLSX |
| GSE133936_BJAB-EBV-3-peaks.xlsx |
3.2 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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