|
| Status |
Public on Jul 27, 2020 |
| Title |
FXR isoform selective transcriptional activation in mouse liver organoids |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The farnesoid X receptor (FXR) is a nuclear receptor activated by bile acids that regulates metabolic processes. FXR is expressed as four isoforms (α1-4), and their relative abundance is specific to tissue and bio-energetic conditions (Correia JC et al. 2015). Depending on the FXR isoform expressed, there is a degree of selectivity in target-genes activation. In this dataset, we defined FXR-isoforms selective effects on transcription in mouse liver organoids after treatment with the FXR agonist Obeticholic acid(OCA). By linking the DNA binding profiles of the FXR isoforms with their transcriptional output, we concluded that differential DNA binding plays a defining role in FXR-isoform target gene selectivity.
|
| |
|
| Overall design |
FXR -/- mouse liver organoids were transduced with mouse FXR isoforms α1, α2, α3, α4 or H2b-NeonGreen(ko) using lentiviral vectors (pLV IRES PuroR). After selection, the organoids were differentiated into the hepatocyte lineage before treatment with OCA
|
| |
|
| Contributor(s) |
Ramos Pittol JM, van Mil SW |
| Citation(s) |
32712104 |
| |
| Submission date |
Jul 02, 2019 |
| Last update date |
Jul 27, 2020 |
| Contact name |
Jose Miguel Ramos Pittol |
| E-mail(s) |
Jose.Ramos-Pittol@uibk.ac.at
|
| Organization name |
University of Innsbruck
|
| Department |
Institute of Biochemistry
|
| Street address |
Innrain 80-82
|
| City |
Innsbruck |
| ZIP/Postal code |
6020 |
| Country |
Austria |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (30)
|
|
| Relations |
| BioProject |
PRJNA552358 |
| SRA |
SRP212799 |
Less...
More...