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| Status |
Public on Jun 30, 2022 |
| Title |
TWIST1 is required for HSC maintenance under steady state and stress condition by inhibiting mitochondrial metabolism gene program |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
RNA-seq and ATAC-seq were performed to determine possible downstream molecular mechamisms and the changes in chromatin accessibility in long-term hematopoietic stem cell when TWIST1 was absent following irradiation stress. We found that mitochondrial metabolism pathway was strongly turned on in the Twist1-deficient LT-HSCs upon irradiation stress.
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| Overall design |
a) RNA-seq in Twist1-deleted and control LT-HSCs following irradiation treatment; b) ATAC-seq in Twist1-deleted and control LT-HSCs following irradiation treatment; c) RNA-seq in Twist1-deleted and control LT-HSCs under steady state.
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| Contributor(s) |
Ma X, Wang N |
| Citation(s) |
33619534 |
| |
| Submission date |
Jul 02, 2019 |
| Last update date |
Jul 01, 2022 |
| Contact name |
Xiao Tong Ma |
| E-mail(s) |
maxt@ihcams.ac.cn
|
| Phone |
+862223909405
|
| Organization name |
Chinese Academy of Medical Sciences
|
| Department |
Institute of Hematology & Blood Diseases Hospital
|
| Lab |
State Key Laboratory of Experimental Hematology
|
| Street address |
Blood hospital, 288 Nanjing Road
|
| City |
TianJin |
| State/province |
TianJin |
| ZIP/Postal code |
300020 |
| Country |
China |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA552226 |
| SRA |
SRP212752 |
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