|
| Status |
Public on Dec 05, 2020 |
| Title |
Reduction of alternative electron acceptors drives biofilm formation in Shewanella algae |
| Organism |
Shewanella algae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Shewanella spp. possess a broad respiratory versatility, which contributes to the occupation of hypoxic/anoxic environmental or host-associated niches. Here we observed a strain-specific induction of biofilm formation in response to supplementation with the anaerobic electron acceptors dimethyl sulfoxide (DMSO) and nitrate in a panel of Shewanella algae isolates. The respiration-driven biofilm response is not observed in DMSO and nitrate reductase deletion mutants of the type strain S. algae CECT 5071, and can be restored upon complementation with the corresponding reductase operon(s) but not by an operon containing a catalytically inactive nitrate reductase. The distinct transcriptional changes, proportional to the effect of these compounds on biofilm formation, include cyclic di-GMP (c-di-GMP) turnover genes. In support, ectopic expression of the c-di-GMP phosphodiesterase YhjH of Salmonella Typhimurium but not its catalytically inactive variant decreased biofilm formation. The respiration-dependent biofilm response of S. algae may permit differential colonization of environmental or host niches.
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| |
|
| Overall design |
Transcriptomics in media with two different electron acceptors, DMSO and nitrate
|
| |
|
| Contributor(s) |
Gonzalez JM |
| Citation(s) |
33504806 |
| |
| Submission date |
Jul 01, 2019 |
| Last update date |
Jan 29, 2021 |
| Contact name |
Jose Gonzalez |
| E-mail(s) |
jmglezh@ull.edu.es
|
| Organization name |
University of La Laguna
|
| Department |
Dept. Microbiology
|
| Street address |
School of Pharmacy
|
| City |
La Laguna |
| ZIP/Postal code |
38200 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL26874 |
Illumina HiSeq 2500 (Shewanella algae) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA552062 |
| SRA |
SRP212705 |
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