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| Status |
Public on Oct 02, 2020 |
| Title |
Transcriptional effects of YAP/TAZ depletion during oncogene-mediated transformation in mouse astrocyte |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To understand the role of YAP/TAZ during gliomagenesis, we compared the transcriptomes of control and YAP/TAZ knockout astrocytes derived from newborn mice, transformed with oncogenic HER2.
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| Overall design |
Yapf/f;Tazf/f mouse astrocyte were expanded and transduced with lentiviral particles encoding for doxy-inducible version of HER2CA, rtTA and CreERT2. Cells were also treated with DMSO (control) or TAM to promote YAP/TAZ knockout during the oncogene transformation. Not transformed astrocytes were used as control. Fully formed P1 Gliomaspheres were also profiled as comparison. After 15 days, cells were harvested for RNA extraction. Gliomaspheres were harvested for RNA extraction 3 weeks after the beginning of the experiment; RNA was then used for polyA RNA-sequencing.
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| Contributor(s) |
Romano O, Bicciato S, Castellan M, Cordenonsi M, Piccolo S |
| Citation(s) |
33644767 |
| |
| Submission date |
Jun 27, 2019 |
| Last update date |
Mar 11, 2021 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
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| Phone |
+39-049-827-6108
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| Organization name |
University of Padova
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| Department |
Molecular Medicine
|
| Street address |
via U. Bassi 59/b
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| GSM3909741 |
Mouse astrocytes HER2CA Day 15 repA [exp1] |
| GSM3909742 |
Mouse astrocytes HER2CA Day 15 repB [exp1] |
| GSM3909743 |
Mouse astrocytes HER2CA TAM Day 15 repA [exp1] |
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| This SubSeries is part of SuperSeries: |
| GSE133471 |
Transcriptional effects of YAP/TAZ depletion in astrocytes, gliomaspheres, and glioblastoma cell lines |
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| Relations |
| BioProject |
PRJNA551496 |
| SRA |
SRP212218 |
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