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| Status |
Public on Apr 10, 2020 |
| Title |
hsa_circ_0007841: A novel potential biomarker and drug resistance for multiple myeloma |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Purpose:It has been shown that circular RNA (circRNA) is a key regulatory factor in development and progression of human tumors. However, the working mechanism and clinical significance of most circRNAs remain unknown in human cancer, including multiple myeloma (MM), a hematologic malignancy. Patients and methods:In the present study, high-throughput circRNA microarray was combined with bioinformatics to identify differentially expressed circRNAs in MM. The hsa_circ_0007841 expressions were shown in MM tissues of 86 patients and drug-resistant cell lines and pathological features were detected. Further, the relationship between hsa_circ_0007841 expressions in the MM tissues and pathological features of MM patients were discussed. The role of hsa_circ_0007841 as a potential biomarker and therapeutic target was analyzed. Results:Our results indicated that in MM tissues collected from patients, MM cell lines and drug-resistant cell lines, the hsa_circ_0007841 expression was significantly upregulated. A close connection with prognosis was observed. That is, hsa_circ_0007841 upregulation was correlated with chromosomal aberrations, such as gain 1q21, t(4:14) and mutations in ATR and IRF4 genes. This finding was further verified in large samples. Finally, bioinformatics analysis indicated interaction that 8 differentially expressed miRNAs and 10 candidate mRNAs interacted with hsa_circ_0007841. These results sheds new light on the basic functional research in the future. Conclusion:It was reported for the first time that hsa_circ_0007841 was significantly upregulated in MM. Our study indicated that hsa_circ_0007841 may be a novel biomarker for MM and involved in the progression of MM.
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| Overall design |
the bone marrow samples from 3 MM patients were sorted by magnetic activated cell sorting (MACS) using anti-CD138 MicroBeads, and the plasma cells were enriched.
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| Contributor(s) |
Gao M, Xiao H, Hang D, Jiang S, Fu Y, Gong L |
| Citation(s) |
31803627 |
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| Submission date |
Jun 20, 2019 |
| Last update date |
Apr 10, 2020 |
| Contact name |
FU YUN FENG |
| E-mail(s) |
14579@qq.com
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| Phone |
13786450644
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| Organization name |
The Third Xiangya Hospital of Central South University
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| Department |
Blood
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| Lab |
Blood
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| Street address |
No. 138 Tonglu Road, Yuelu District, Hexi Distric
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| City |
CHANG SHA |
| State/province |
HUNAN |
| ZIP/Postal code |
410000 |
| Country |
China |
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| Platforms (1) |
| GPL21825 |
074301 Arraystar Human CircRNA microarray V2 |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA549859 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133058_RAW.tar |
4.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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