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| Status |
Public on Jul 09, 2020 |
| Title |
Analysis of exosomal circRNAs upon irradiation in pancreatic cancer cell repopulation |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Next generation sequencing (NGS) and bioinformatics were performed to analysis and annotate the expression profiles and functions of exosome-derived circRNAs in pancreatic cells during radiation. A total of 12572 circRNAs were identified, and 3580 circRNAs were annotated in literatures and circBase. There were 196 filtered differentially expressed circRNAs (182 for up-regulated and 14 for down-regulated, fold change > 2, p-value < 0.05). GO and KEGG analyses indicated that regulation of metabolic process and lysine degradation were the main biological processes and pathway enrichment. 4 circRNAs were chosen for qRT-PCR analysis to validate the sequencing. The hsa_circ_0002130-hsa-miR-4482-3p-NBN interaction network suggested potential miRNA sponges and target mRNAs. Our results provided potential functions of circRNAs to explore molecular mechanisms and therapeutic targets in pancreatic cancer cell upon irradiation.
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| |
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| Overall design |
Examination of total RNA profiles of pancreatic cells-derived exosomes by NGS
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| |
|
| Contributor(s) |
Tian L |
| Citation(s) |
32727565 |
| |
| Submission date |
Jun 13, 2019 |
| Last update date |
Oct 10, 2020 |
| Contact name |
Ling Tian |
| E-mail(s) |
TL09168@hotmail.com
|
| Organization name |
Shanghai General Hospital
|
| Department |
Institute of Translational Medicine
|
| Lab |
Shanghai Key Laboratory of Pancreatic Diseases
|
| Street address |
No. 650, New Songjiang Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
201620 |
| Country |
China |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA548744 |
| SRA |
SRP201326 |
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