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Series GSE132562 Query DataSets for GSE132562
Status Public on Feb 21, 2020
Title A reversible KO model reveals therapeutic potentials of defective Tregs
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To characterize Brg1-reexpressed Tregs, we treated 3-wks-old rKO1 mice with the low dose of TAM and compared gene expression patterns in Brg1-deleted (GFP+) vs. Brg1-reexpressed (GFP- ) Treg subsets isolated 7 days after TAM.
Brg1-deletion in the mosaic females and Brg1-reexpression in rKO1 mice on Day 7 after TAM treatment affected 618 and 1352 genes, respectively, with only 241 genes shared, suggesting divergent roles of Brg1 under the physiological vs. inflammatory conditions. These genes can be divided into two categories: the “naïve genes” that are predominantly expressed in naïve Tregs and “activation/effector function genes” preferentially expressed in activated/effector Tregs.
Our data collectively suggest that Brg1 reexpression acted (partly) in conjunction with inflammatory cytokines to convert Brg1-deleted Tregs into hyperactivated Tregs endowed with potent suppressive activity
Overall design mRNA profiles of 3-4 weeks old wild type (WT) and Nrl-/- mice were generated by deep sequencing, in triplicate, using HiSeq X Ten. BioProject: PRJNA547476
Contributor(s) Li Y, Chen Y, Mao S, Kaundal R, Jing Z, Chen Q, Wang X, Xia J, Sun J, Wang H, Chi T
Citation(s) 32493900
BioProject PRJNA547476
Submission date Jun 11, 2019
Last update date Jun 08, 2020
Contact name Zhengyu Jing
Organization name ShanghaiTech University
Department School of Life Science and Technology
Lab Chi Lab
Street address 393 Middle Huaxia Road, Pudong
City Shanghai
ZIP/Postal code 201210
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (8)
GSM3874705 WT
GSM3874706 Brg1 expressing subset of tregs from mosaic females
GSM3874707 Brg1 deleted subset of tregs from mosaic females
SRA SRP200611

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Supplementary file Size Download File type/resource
GSE132562_RAW.tar 2.3 Mb (http)(custom) TAR (of TXT)
GSE132562_count.xlsx 5.8 Mb (ftp)(http) XLSX
GSE132562_gene_fpkm.xlsx 7.0 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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