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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 21, 2020 |
| Title |
A reversible KO model reveals therapeutic potentials of defective Tregs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To characterize Brg1-reexpressed Tregs, we treated 3-wks-old rKO1 mice with the low dose of TAM and compared gene expression patterns in Brg1-deleted (GFP+) vs. Brg1-reexpressed (GFP- ) Treg subsets isolated 7 days after TAM.
Brg1-deletion in the mosaic females and Brg1-reexpression in rKO1 mice on Day 7 after TAM treatment affected 618 and 1352 genes, respectively, with only 241 genes shared, suggesting divergent roles of Brg1 under the physiological vs. inflammatory conditions. These genes can be divided into two categories: the “naïve genes” that are predominantly expressed in naïve Tregs and “activation/effector function genes” preferentially expressed in activated/effector Tregs.
Our data collectively suggest that Brg1 reexpression acted (partly) in conjunction with inflammatory cytokines to convert Brg1-deleted Tregs into hyperactivated Tregs endowed with potent suppressive activity
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| Overall design |
mRNA profiles of 3-4 weeks old wild type (WT) and Nrl-/- mice were generated by deep sequencing, in triplicate, using HiSeq X Ten. BioProject: PRJNA547476
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| Contributor(s) |
Li Y, Chen Y, Mao S, Kaundal R, Jing Z, Chen Q, Wang X, Xia J, Sun J, Wang H, Chi T |
| Citation(s) |
32493900 |
| BioProject |
PRJNA547476 |
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| Submission date |
Jun 11, 2019 |
| Last update date |
Jun 08, 2020 |
| Contact name |
Zhengyu Jing |
| E-mail(s) |
jingzhy@shanghaitech.edu.cn
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| Organization name |
ShanghaiTech University
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| Department |
School of Life Science and Technology
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| Lab |
Chi Lab
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| Street address |
393 Middle Huaxia Road, Pudong
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| City |
Shanghai |
| ZIP/Postal code |
201210 |
| Country |
China |
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| Platforms (1) |
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| Samples (8)
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| GSM3874708 |
Brg1 deleted tregs from rko1 mice |
| GSM3874709 |
Brg1 deleted tregs subset from rko1 mice, isolated on day 7 after tam |
| GSM3874710 |
Brg1 reexperssed tregs subset from rko1 mice, isolated on day 7 after tam |
| GSM4115626 |
Brg1 deleted aTregs subset from rKO1 mice, isolated on day 7 after tam |
| GSM4115627 |
Brg1 reexperssed atregs subset from rko1 mice, isolated on day 7 after tam |
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| Relations |
| SRA |
SRP200611 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE132562_RAW.tar |
2.3 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE132562_count.xlsx |
5.8 Mb |
(ftp)(http) |
XLSX |
| GSE132562_gene_fpkm.xlsx |
7.0 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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