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Series GSE132446 Query DataSets for GSE132446
Status Public on Feb 12, 2020
Title Refined spatial temporal epigenomic profiling reveals intrinsic connection between PRDM9-mediated H3K4me3 and the fate of double-stranded breaks
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Meiotic recombination is initiated by the formation of double-strand breaks (DSBs), which are repaired as either crossovers (COs) or noncrossovers (NCOs). In most mammals, PRDM9-mediated H3K4me3 controls the nonrandom distribution of DSBs; however, both the timing and mechanism of DSB fate control remain largely undetermined. Here, we generated comprehensive epigenomic profiles of synchronized mouse spermatogenic cells during meiotic prophase I, revealing spatiotemporal and functional relationships between epigenetic factors and meiotic recombination. We find that PRDM9-mediated H3K4me3 at DSB hotspots, coinciding with H3K27ac and H3K36me3, is intimately connected with the fate of the DSB. Our data suggest that the fate decision is likely made at the time of DSB formation: earlier formed DSBs occupy more open chromatins and are much more competent to proceed to a CO fate. Our work highlights an intrinsic connection between PRDM9-mediated H3K4me3 and the fate decision of DSBs, and provides new insight into the control of CO homeostasis.
Overall design We have applied chromatin ChIP-seq (Chromatin immunoprecipitation and sequencing) to spermatogenic cells for six critical histone modifications, including H3K4me3, H3K9me2, H3K9me3, H3K27me3, H3K36me3, and H3K27ac, and NOMe-seq (Nucleosome Occupancy and Methylome Sequencing) for the chromatin state, nucleosome positioning, and DNA methylation for each sample with two biological replicates. We also performed ChIP-seq and NOMe-seq for synchronous leptotene and zygotene from Prdm9-/-, Spo11-/-, and Dmc1-/- mice.
Examination of 6 different histone modifications in homogenous synchronous spermatogenic cells of 8 stages during meiotic process.
Contributor(s) Chen Y, Lyu R, Rong B, Zheng Y, Lin Z, Dai R, Zhang X, Xie N, Wang S, Tang F, Lan F, Tong M
Citation(s) 32047271
Submission date Jun 10, 2019
Last update date Apr 20, 2021
Contact name Yuxuan Zheng
Organization name Fudan University
Street address 825 Zhangheng Rd.
City Shanghai
ZIP/Postal code 201203
Country China
Platforms (3)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21273 HiSeq X Ten (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (186)
GSM3864886 Undiff_1_combined
GSM3864887 Undiff_2_combined
GSM3864888 A1_1_combined
BioProject PRJNA548107
SRA SRP200968

Download family Format
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Supplementary file Size Download File type/resource
GSE132446_RAW.tar 62.5 Gb (http)(custom) TAR (of BED, BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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