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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 30, 2020 |
| Title |
Next Generation Sequencing timecourse of murine hepatocytes with or without paracetamol treatment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Untreated mice or mice treated with paracetamol, in the presence or absence of TSA, were culled after 14 hrs or 24 hrs of treatment, and RNAseq was performed.
Method: RNA was extracted, in triplicate, then sequenced with an Illumina NextSeq 500 sequencer. Sequence reads passing the quality control filters were aligned using Tophat2 and then analysed with Cufflinks.
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| Overall design |
Quadruplicate polyA mRNA profiles from mouse hepatocytes were generated following various drug treatment protocols, using an Illumina NextSeq 500 sequencer.
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| Contributor(s) |
Vizioli M, Adams PD, Gilroy K |
| Citation(s) |
32001510 |
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| Submission date |
Jun 07, 2019 |
| Last update date |
Jul 01, 2020 |
| Contact name |
Xue Lei |
| E-mail(s) |
xlei@sbpdiscovery.org
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| Organization name |
SBP medical discovery institute
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| Lab |
Peter Adams Lab
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| Street address |
10901 N Torrey Pines Rd
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (24)
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| GSM3861003 |
Hepatocytes, DMSO 0hr Rep4 |
| GSM3861004 |
Hepatocytes, TSA 0hr Rep1 |
| GSM3861005 |
Hepatocytes, TSA 0hr Rep2 |
| GSM3861006 |
Hepatocytes, TSA 0hr Rep3 |
| GSM3861007 |
Hepatocytes, TSA 0hr Rep4 |
| GSM3861008 |
Hepatocytes, ParaDMSO 14hr Rep1 |
| GSM3861009 |
Hepatocytes, ParaDMSO 14hr Rep2 |
| GSM3861010 |
Hepatocytes, ParaDMSO 14hr Rep3 |
| GSM3861011 |
Hepatocytes, ParaDMSO 14hr Rep4 |
| GSM3861012 |
Hepatocytes, ParaTSA 14hr Rep1 |
| GSM3861013 |
Hepatocytes, ParaTSA 14hr Rep2 |
| GSM3861014 |
Hepatocytes, ParaTSA 14hr Rep3 |
| GSM3861015 |
Hepatocytes, ParaTSA 14hr Rep4 |
| GSM3861016 |
Hepatocytes, ParaDMSO 24hr Rep1 |
| GSM3861017 |
Hepatocytes, ParaDMSO 24hr Rep2 |
| GSM3861018 |
Hepatocytes, ParaDMSO 24hr Rep3 |
| GSM3861019 |
Hepatocytes, ParaDMSO 24hr Rep4 |
| GSM3861020 |
Hepatocytes, ParaTSA 24hr Rep1 |
| GSM3861021 |
Hepatocytes, ParaTSA 24hr Rep2 |
| GSM3861022 |
Hepatocytes, ParaTSA 24hr Rep3 |
| GSM3861023 |
Hepatocytes, ParaTSA 24hr Rep4 |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA547765 |
| SRA |
SRP200758 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE132368_RAW.tar |
24.8 Mb |
(http)(custom) |
TAR (of FPKM_TRACKING) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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