GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE132254 Query DataSets for GSE132254
Status Public on Sep 17, 2019
Title RNA Seq analyzed the transcriptional difference between the ICSI and ICAHCI embryo in blastocyst and placenta
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Our lab first derived mouse androgenetic haploid embryonic stem cells (AG-haESCs) and demonstrated that AG-haESCs can be used as an “artificial spermatids” to generate gene-edited semi-cloned (SC) mice through intracytoplasmic injection (ICAHCI) into mature oocyte, even though the birth efficiency is very low. Further we proved that H19-DMR and IG-DMR were the main barrier to generate viable mice through androgenetic and parthenogenetic haESCs. More importantly, AG-haESCs mediated SC technology combined with CRISPR-Cas9 is a powerful tool to generate gene-modified mouse models and carry out genetic screening at organismal level. However, it is still not clear how the H19-DMR and IG-DMR coordinately regulate SC embryo development. Here, we found that the H19-DMR and IG-DMR regulate the development of SC embryos in spatio-temporal scales. Firstly, we found that the H19-DMR and IG-DMR are not indispensable for the development of preimplantation of SC embryos. Secondly, H19-DMR is essential for the development of SC embryos in mid-gestation and IG-DMR takes effect in late-gestation. Further, the maintenance of paternal H19-DMR methylation status and deletion of paternal H19 transcription unit play a key role in the structures and transport functions of SC embryo placenta. Importantly, AG-haESCs carrying triple deletions, including H19, H19-DMR and IG-DMR, can further improve the efficiency in generation of viable, normal-size, and fertile mice.
Overall design For blastocyst, five samples (including ICM and TE) for control (ICSI) and semi-cloned blastocysts (including WT and DKO). For placenta, three samples for control (ICSI) and semi-cloned placentae (including WT, DKO, IG△DMR and H19△13kb)
Contributor(s) Li J, Li Q, Li Y
Citation(s) 31564034
Submission date Jun 05, 2019
Last update date Oct 13, 2019
Contact name Qing Li
Organization name Chinese Academy of Science
Department institute of biochemistry and cell biology
Lab Jinsong Li's Lab
Street address 320 Yueyang Road
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (2)
GPL21273 HiSeq X Ten (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (45)
GSM3854960 ICSI_ICM_RNA seq_1
GSM3854961 ICSI_ICM_RNA seq_2
GSM3854962 ICSI_ICM_RNA seq_3
BioProject PRJNA546605
SRA SRP200552

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE132254_RAW.tar 6.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap