|
| Status |
Public on Aug 06, 2019 |
| Title |
LSD1 regulates the plasmablast transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand the role of LSD1 in marginal zone B cell development, CD93+ transitional B cells were enriched from the spleens of mice with B cell conditional deletion of LSD1, the cells were cultured for 3 days in the presence of OP9-DL1 cells and BAFF, and day 0 B220+CD93+ transitional B cells and day 3 B220+CD21+CD1d+ ex vivo-derived marginal zone B cells were FACS-sorted and RNA-seq was performed to identify LSD1-target regulated genes.
|
| |
|
| Overall design |
RNA-seq on control or LSD1-deficient murine splenic transitional B cells and ex vivo-derived marginal zone B cells
|
| |
|
| Contributor(s) |
Haines RR, Scharer CD, Boss JM |
| Citation(s) |
31492745 |
| |
| Submission date |
Jun 04, 2019 |
| Last update date |
Nov 05, 2019 |
| Contact name |
Jeremy Matthew Boss |
| E-mail(s) |
jeremy.boss@emory.edu
|
| Organization name |
Emory University
|
| Department |
Microbiology and Immunology
|
| Lab |
Dr. Jeremy M. Boss
|
| Street address |
1510 Clifton Rd NE, Room 3120
|
| City |
Atlanta |
| State/province |
Georgia |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (12)
|
|
| This SubSeries is part of SuperSeries: |
| GSE132227 |
LSD1 cooperates with non-canonical NF-êB signaling to regulate marginal zone B cell development |
|
| Relations |
| BioProject |
PRJNA546438 |
| SRA |
SRP200437 |
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