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Series GSE132184 Query DataSets for GSE132184
Status Public on Sep 01, 2019
Title Single-cell RNA transcriptome of induced noradrenergic (iNA) neurons from astrocytes and mouse embryonic fibroblasts
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We reported the generation of iNA neurons from astrocytes and MEFs by direct cell reprogramming. To compare the transcriptome of iNA neurons induced from this two kinds of starter cells, we prepared the single-cell cDNA library of iNA neurons induced from both starter cells in vitro for 28 days by 10x Genomics. Our results show that the single-cell RNA transcriptome of iNA neurons induced from astrocytes and MEFs is similar.
Overall design Control astrocytes, MEFs, mCherry+ induced neurons at 28 days in vitro generated from astrocytes and MEFs of Dbh-2a-mCherry mice were enriched by FACS and put to library construction by 10x Genomics. The single-cell cDNA library were sequenced using Hiseq 6000 platform.
Contributor(s) Li S, Zhou Z, Shen L, Cheng L
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Submission date Jun 04, 2019
Last update date Sep 03, 2019
Contact name Sanlan Li
Organization name Institute of Neuroscience, Chinese Academy of Sciences
Street address 320 Yueyang Road, Xuhui District
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM3852665 FUGW_1
GSM3852666 F7_1
GSM3852667 MF
BioProject PRJNA546271
SRA SRP200412

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Supplementary file Size Download File type/resource
GSE132184_RAW.tar 622.7 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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