|
| Status |
Public on Sep 01, 2019 |
| Title |
Single-cell RNA transcriptome of induced noradrenergic (iNA) neurons from astrocytes and mouse embryonic fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We reported the generation of iNA neurons from astrocytes and MEFs by direct cell reprogramming. To compare the transcriptome of iNA neurons induced from this two kinds of starter cells, we prepared the single-cell cDNA library of iNA neurons induced from both starter cells in vitro for 28 days by 10x Genomics. Our results show that the single-cell RNA transcriptome of iNA neurons induced from astrocytes and MEFs is similar.
|
| |
|
| Overall design |
Control astrocytes, MEFs, mCherry+ induced neurons at 28 days in vitro generated from astrocytes and MEFs of Dbh-2a-mCherry mice were enriched by FACS and put to library construction by 10x Genomics. The single-cell cDNA library were sequenced using Hiseq 6000 platform.
|
| |
|
| Contributor(s) |
Li S, Zhou Z, Shen L, Cheng L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 04, 2019 |
| Last update date |
Sep 03, 2019 |
| Contact name |
Sanlan Li |
| Organization name |
Institute of Neuroscience, Chinese Academy of Sciences
|
| Street address |
320 Yueyang Road, Xuhui District
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA546271 |
| SRA |
SRP200412 |
Less...
More...