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| Status |
Public on Jul 15, 2019 |
| Title |
Associated bacteria affect sexual reproduction by altering gene expression and metabolic processes in a biofilm inhabiting diatom (Seminavis robusta) |
| Organism |
Seminavis robusta |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) of Seminavis robusta in presence and absence of associated bacterial spent medium (Maribacter sp. and Roseovarius sp.) in order to highlight the effect of bacterial exudates on diatom gene expression and metabolic processes.
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| Overall design |
Transcriptomics of 6 different treatments of Seminavis robusta mating type - (MT-) treated with spent medium of associated bacteria (Roseovarius sp. and Maribacter sp.). In three treatments S. robusta was sexually induced (SIP, SIPM and SIPR), in other three treatments it was not sexually induced (C, M, R). Differential gene expression was employed to find genes influenced either by the induction fo sexuality, by the presence of bacteria or by both effects.
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| Contributor(s) |
Cirri E, De Decker S |
| Citation(s) |
31428077 |
| |
| Submission date |
May 23, 2019 |
| Last update date |
Oct 20, 2019 |
| Contact name |
Emilio Cirri |
| E-mail(s) |
emilio.cirri@gmail.com
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| Organization name |
Friedrich Schiller University Jena
|
| Street address |
Lessingstrasse 8
|
| City |
Jena |
| ZIP/Postal code |
07743 |
| Country |
Germany |
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| Platforms (1) |
| GPL26704 |
Illumina NextSeq 500 (Seminavis robusta) |
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| Samples (18)
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| GSM3814780 |
Sexaully Induced S. robusta MT- (SIP) rep1 |
| GSM3814781 |
Sexaully Induced S. robusta MT- (SIP) rep2 |
| GSM3814782 |
Sexaully Induced S. robusta MT- (SIP) rep3 |
| GSM3814783 |
S. robusta MT- + Roseovarius ( R) rep1 |
| GSM3814784 |
S. robusta MT- + Roseovarius ( R) rep2 |
| GSM3814785 |
S. robusta MT- + Roseovarius ( R) rep3 |
| GSM3814786 |
S. robusta MT- + Maribacter (M) rep1 |
| GSM3814787 |
S. robusta MT- + Maribacter (M) rep2 |
| GSM3814788 |
S. robusta MT- + Maribacter (M) rep3 |
| GSM3814789 |
Sexaully Induced S. robusta MT- + Maribacter (SIPM) rep1 |
| GSM3814790 |
Sexaully Induced S. robusta MT- + Maribacter (SIPM) rep2 |
| GSM3814791 |
Sexaully Induced S. robusta MT- + Maribacter (SIPM) rep3 |
| GSM3814792 |
Sexaully Induced S. robusta MT- + Roseovarius (SIPR) rep1 |
| GSM3814793 |
Sexaully Induced S. robusta MT- + Roseovarius (SIPR) rep2 |
| GSM3814794 |
Sexaully Induced S. robusta MT- + Roseovarius (SIPR) rep3 |
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| Relations |
| BioProject |
PRJNA544526 |
| SRA |
SRP199371 |
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