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| Status |
Public on Feb 20, 2020 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes of Murine Fibroblast Fibroproliferative Response with TGFβ |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
AKR-2B cells were grown in an anchorage independent system to assess transcriptional profile of the TGFβ-dependent fibroproliferative response.
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| |
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| Overall design |
AKR-2B cells were grown in soft agar -/+TGFβ, and colonies isolated and total RNA purified at 2, 4, and 8 days, then sent for NGS of mRNA and mRNA, and analyzed for the differentially increased genes associated with TGFβ stimulation.
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| |
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| Contributor(s) |
Leof EB, Hernandez DM, Yin X |
| Citation(s) |
32067272 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM054200 |
TGF beta Receptors and Cell Proliferation |
MAYO CLINIC |
EDWARD B LEOF |
| R37 GM055816 |
TGF Beta Receptor Dynamics |
MAYO CLINIC |
EDWARD B LEOF |
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| |
| Submission date |
May 15, 2019 |
| Last update date |
Feb 20, 2020 |
| Contact name |
Edward B. Leof |
| Organization name |
Mayo Clinic
|
| Department |
Thoracic Disease Research Unit
|
| Lab |
Stabile 8-52
|
| Street address |
200 First Street SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA543065 |
| SRA |
SRP198476 |
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