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Series GSE131246 Query DataSets for GSE131246
Status Public on Jul 27, 2019
Title Gene expression analysis of R1 mESCs stably expressing WT ESRRB and Ser 25A (the O-GlcNAc residue mutant) ESRRB
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Unnatural monosaccharides such as azidosugars that can be metabolically incorporated into cellular glycans are currently used as a major tool for glycan imaging and glycoproteomic profiling. As a common practice to enhance membrane permeability and cellular uptake, the unnatural sugars are per O-acetylated, which, however, can induce a long-overlooked side reaction, non enzymatic S-glycosylation. Herein, we develop 1,3-di-esterified N azidoacetylgalactosamine (GalNAz) as the next generation chemical reporters for metabolic glycan labeling. Both 1,3-di-O-acetylated GalNAz (1,3-Ac2GalNAz) and 1,3-di-O propionylated GalNAz (1,3-Pr2GalNAz) exhibited high efficiency for labeling protein O-GlcNAcylation with no artificial S-glycosylation. Applying 1,3-Pr2GalNAz in mouse embryonic stem cells (mESCs), we identified ESRRB, a critical transcription factor for pluripotency, as an O-GlcNAcylated protein. We showed that ESRRB O-GlcNAcylation is important for mESC self-renewal and pluripotency. Mechanistically, ESRRB is O-GlcNAcylated by O-GlcNAc transferase (OGT) at serine 25 (Ser 25), which stabilizes ESRRB, promotes its transcription activity and facilitates its interactions with two master pluripotency regulators, OCT4 and NANOG.
Overall design Three biological replicates of R1 embryonic stem cells stably expressing WT ESRRB and Ser 25A ESRRB were analysed.
Contributor(s) Zhang C, Hao Y, Chen X
Citation(s) 31492838
Submission date May 15, 2019
Last update date Sep 18, 2019
Contact name Xing Chen
Organization name Peking University
Department Chemical Biology
Street address Yiheyuan Road
City Beijing
ZIP/Postal code 100871
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM3768056 WT-1 RNA-seq
GSM3768057 WT-2 RNA-seq
GSM3768058 WT-3 RNA-seq
BioProject PRJNA543040
SRA SRP198469

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Supplementary file Size Download File type/resource
GSE131246_counts.txt.gz 3.9 Mb (ftp)(http) TXT
GSE131246_deseq.csv.gz 1.3 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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