|
| Status |
Public on Sep 03, 2019 |
| Title |
The grain yield modulator miR156 regulates seed dormancy through the gibberellin pathway in rice |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The widespread agricultural problem of pre-harvest sprouting (PHS) could potentially be overcome by improving seed dormancy. Here, we report that miR156, an important grain yield regulator, also controls seed dormancy in rice. We found that mutations in one MIR156 subfamily enhance seed dormancy and suppress PHS with negligible effects on shoot architecture and grain size, whereas mutations in another MIR156 subfamily modify shoot architecture and increase grain size but have minimal effects on seed dormancy. Mechanistically, mir156 mutations enhance seed dormancy by suppressing the gibberellin (GA) pathway through de-represssion of the miR156 target gene Ideal Plant Architecture 1 (IPA1), which directly regulates multiple genes in the GA pathway. These results provide an effective method to suppress PHS without compromising productivity, and will facilitate breeding elite crop varieties with ideal plant architectures.
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| |
|
| Overall design |
High-depth paired-end RNA-seq from Nipponbare (the wild type), mir156abckl, mir156defghi and mir156abcdfghikl mutant rice. Each sample contains 3 biological replicates.
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| |
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| Contributor(s) |
Miao C, Wang Z, Zhu J |
| Citation(s) |
31444356 |
| |
| Submission date |
May 15, 2019 |
| Last update date |
Sep 03, 2019 |
| Contact name |
Jian-Kang Zhu |
| E-mail(s) |
jkzhu@sibs.ac.cn
|
| Organization name |
Shanghai Center for Plant Stress Biology
|
| Street address |
No. 3888 Chenhua Road
|
| City |
Shanghai |
| ZIP/Postal code |
201602 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL19290 |
Illumina HiSeq 2500 (Oryza sativa) |
|
| Samples (7)
|
| GSM3768021 |
Unelongated culm of 20-day-old wild-type seedlings |
| GSM3768022 |
Unelongated culm of 20-day-old mir156abckl seedlings [D183] |
| GSM3768023 |
Unelongated culm of 20-day-old mir156defghi seedlings [D265] |
|
| Relations |
| BioProject |
PRJNA543036 |
| SRA |
SRP198467 |
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