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| Status |
Public on Feb 17, 2021 |
| Title |
RNA-seq of murine primary adult stem cells of Trrap inducible knockout Mus musculus with and without 4-OHT treatment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The acetylation levels of histones and other proteins change during aging and have been linked to neurodegeneration. Here we show that deletion of the histone acetyltransferase (HAT) co-factor Trrap specifically impairs the function of the transcription factor Sp1, reduces its stability and causes a decrease in histone acetylation at Sp1 target genes. Modulation of Sp1 function by Trrap acts as a hub regulating multiple processes involved in neuron and neural stem cells function and maintenance including microtubule dynamics and the Wnt signaling pathway. Consistently, Trrap conditional mutants exhibit all hallmarks of neurodegeneration including dendrite retraction and axonal swellings, neuron death, astrogliosis, microglia activation, demyelination and decreased adult neurogenesis. Our results uncovered a novel functional network, essential to prevent neurodegeneration, and involving the specific regulation of Sp1 transcription factor and its downstream targets by Trrap-HAT.
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| Overall design |
15 samples in total:
5 with no treatment (Trrap Fl/+; Rosa26-CreERT2 tg)
5 with 4-OHT treatment (Trrap Fl/+; Rosa26-CreERT2 tg)
5 with 4-OHT treatment (Trrap Fl/Fl; Rosa26-CreERT2 tg)
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| |
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| Contributor(s) |
Groth M, Koch P, Pellón DL, Wang Z |
| Citation(s) |
33594975 |
| |
| Submission date |
May 14, 2019 |
| Last update date |
May 19, 2021 |
| Contact name |
Philipp Koch |
| Organization name |
Leibniz Institute on Aging - Fritz Lipmann Institute
|
| Department |
Core Facility Life Science Computing
|
| Street address |
Beutenbergstraße 11
|
| City |
Jena |
| ZIP/Postal code |
07745 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (15)
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|
| Relations |
| BioProject |
PRJNA542923 |
| SRA |
SRP198406 |
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