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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 31, 2020 |
| Title |
RNA sequencing analysis of wildtye and Acc2 iKO mice heart in chow and HFD |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here, we show that short term HFD feeding did not change the cardiac function in normal (Con) and increased FAO mice (ACC2 iKO). RNA sequencing analysis show that ACC2 can have small but significant genetic perturbations impact on the global transcriptome under chow and HFD condition. Most of fatty acid degradation and PPAR signaling pathway related gene transcription levels were decreased by knocking out ACC2. Intriguingly, cardiac transcriptome analysis of several lipotoxicity mouse models showed an opposite regulation direction of PPAR signaling pathway and fatty acid degradation genes. Finally, fatty acid degradation gene transcription was found back to normal in iKO-HFD mouse hearts compared to Con-chow mice hearts. These results suggest that fatty acid availability may play an important role in PPAR signaling regulation regardless the fatty acid oxidation rate, at least in mice heart.
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| Overall design |
ACC2 flox/flox-MerCreMer+ (ACC2-f/f-MCM+) mice were mated with ACC2f/f to produce both study and control littermates. At 8 weeks of age, both ACC2f/f-MCM+ and ACC2f/f (CON) mice received an intraperitoneal injection of tamoxifen (20 mg/kg) for 5 days, which was sufficient to cause ACC2 deletion in ACC2f/f-MCM+ (iKO). Four weeks after the last injection of tamoxifen, male CON and iKO mice were subjected to chow or High fat diet for 16 weeks. Illumina HiSeq 2000 at a read length of 100nt single end.
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| Contributor(s) |
Liu Z, Shao D, Tian R |
| Citation(s) |
32592696 |
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| Submission date |
May 13, 2019 |
| Last update date |
Jun 30, 2020 |
| Contact name |
Rong Tian |
| E-mail(s) |
rongtian@uw.edu
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| Organization name |
University of Washington
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| Street address |
850 Republican Street, Room N130
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| City |
SEATTLE |
| State/province |
WA |
| ZIP/Postal code |
98105 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (20)
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| GSM3764475 |
Heart, ACC2 iKO, control, RT4 |
| GSM3764476 |
Heart, WT, control, RT5 |
| GSM3764477 |
Heart, ACC2 iKO, control, RT6 |
| GSM3764478 |
Heart, ACC2 iKO, control, RT7 |
| GSM3764479 |
Heart, WT, control, RT8 |
| GSM3764480 |
Heart, ACC2 iKO, control, RT9 |
| GSM3764481 |
Heart, WT, control, RT10 |
| GSM3764482 |
Heart, ACC2 iKO, HFD, RT11 |
| GSM3764483 |
Heart, WT, HFD, RT12 |
| GSM3764484 |
Heart, ACC2 iKO, HFD, RT13 |
| GSM3764485 |
Heart, WT, HFD, RT14 |
| GSM3764486 |
Heart, ACC2 iKO, HFD, RT15 |
| GSM3764487 |
Heart, WT, HFD, RT16 |
| GSM3764488 |
Heart, ACC2 iKO, HFD, RT17 |
| GSM3764489 |
Heart, WT, HFD, RT18 |
| GSM3764490 |
Heart, WT, HFD, RT19 |
| GSM3764491 |
Heart, ACC2 iKO, HFD, RT20 |
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| Relations |
| BioProject |
PRJNA542673 |
| SRA |
SRP198278 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE131122_RAW.tar |
13.2 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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