|
| Status |
Public on Jun 01, 2019 |
| Title |
RNA-sequencing of human lymph node and peripheral blood T follicular helper cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Total mRNA-sequencing on memory T helper cell populations from human blood and lymph nodes.
|
| |
|
| Overall design |
Paired blood and lymph node samples were taken from patients recruited from the renal transplant live donor program at Cambridge University Hospitals NHS Foundation Trust, and who provided informed consent. All patients were either receiving or within 6 months of requiring renal replacement therapy. Patients taking immunosuppressive medication prior to transplant were excluded. mRNA was isolated from flow sorted CD4+ T cell populations and RNA-sequencing performed.
|
| |
|
| Contributor(s) |
Linterman M, Pierson W, Hill D, Carr E, Wingett S |
| Citation(s) |
31175140 |
| |
| Submission date |
May 13, 2019 |
| Last update date |
Aug 20, 2019 |
| Contact name |
Felix Krueger |
| E-mail(s) |
fkrueger@altoslabs.com
|
| Organization name |
Altos Labs
|
| Department |
Bioinformatics
|
| Street address |
Granta Park
|
| City |
Cambridge |
| ZIP/Postal code |
CB21 6GP |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (44)
|
|
| Relations |
| BioProject |
PRJNA542639 |
| SRA |
SRP198243 |
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