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| Status |
Public on Nov 18, 2019 |
| Title |
Global skin gene expression profile of early diffuse cutaneous systemic sclerosis shows a prominent innate and adaptive immunity profile |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA from skin biopsies from 48 patients in the Prospective Registry for Early Systemic Sclerosis (PRESS) cohort (mean disease duration 1.3 years) and 33 matched healthy controls was examined by nextGen RNA sequencing
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| Overall design |
3- or 4-mm diameter punch biopsies were obtained from the forearm skin and immersed in RNAlater solution (Qiagen). RNA was extracted using miRNeasy Mini kits (Qiagen). cDNA libraries were prepared using the Illumina TruSeq stranded Total RNA Library Prep Gold kit, loaded on cBot (Illumina) at a final concentration of 10 pM to perform cluster generation, followed by 2 x 76 bp paired-end sequencing on HiSeq 2500 (Illumina), generating on average around 50 million reads per sample.
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| Contributor(s) |
Skaug B, Assassi S, Zhu L, Zheng WJ |
| Citation(s) |
31767698, 32603313 |
| |
| Submission date |
May 09, 2019 |
| Last update date |
Jun 02, 2024 |
| Contact name |
Shervin Assassi |
| E-mail(s) |
Shervin.Assassi@uth.tmc.edu
|
| Phone |
7135006900
|
| Organization name |
University of Texas Health Science Center at Houston
|
| Street address |
6431 FanninSuite 5270
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (91)
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| Relations |
| BioProject |
PRJNA542095 |
| SRA |
SRP197303 |
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