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Series GSE1309 Query DataSets for GSE1309
Status Public on Jun 08, 2004
Title Gene expession profiles for monoclonal cell lines with high or low levels of store-operated Ca2+ entry.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This series of microarray experiments monitored the gene expression profiles for monoclonal cell lines (derived from HEK-293 parental cell culture) with high (H1, H15, H24, H36, H39) or low (L3, L28, L29) levels of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from their growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip. Two parallel hybridizations were done for each RNA preparation from each monoclonal cell line or from the parental HEK-293 cell culture.

Keywords: parallel sample
Contributor(s) Zagranichnaya TK, Wu X, Danos A, Villereal ML
Citation(s) 15623568
Submission date Apr 14, 2004
Last update date Aug 10, 2018
Contact name Tatiana Zagranichnaya
Phone 773-702-9337
Organization name The University of Chicago
Street address
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (22)
GSM21234 HEK-293_clone H1_exp #1
GSM21235 HEK-293_clone H1(duplicate)_exp #1
GSM21258 HEK-293_clone H39_exp #1
BioProject PRJNA89905

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