|
| Status |
Public on May 05, 2020 |
| Title |
Next Generation Sequencing Analysis of livers from 2-month WT/12-month WT and 2-month HKO/12-month nontumor/12month- tumor. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
SRSF2 flanked with flox was specifically depleted in the livers by Alb-Cre(HKO),and took the intact ones as contorl(WT).Near 70% of the HKO livers spontaneously developed malignant tumors(took the tumor areas as tumor while the tumor-adjacent as nontumor).Then total RNAs of 2-month WT/HKO and 12-month WT/nontumor/tumor were isolated and performed the next generation sequencing. We profiled the gene expression regulated by Cre-loxp-mediated knockout system.
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| |
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| Overall design |
mRNA profiles of w8-WT80,w8-HKO82,M13-WT44,M13-HKO24,M13-tumor24 liver samples were generated by deep sequencing.
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| |
|
| Contributor(s) |
Zhang C |
| Citation(s) |
32372053 |
| |
| Submission date |
May 06, 2019 |
| Last update date |
May 18, 2020 |
| Contact name |
Chang Zhang |
| E-mail(s) |
zhangchang_08@126.com
|
| Phone |
13262543670
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Shanghai Institute of Nutrition and Health
|
| Lab |
FengYing Lab
|
| Street address |
320 Yue Yang Rd, Xuhui District
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (5)
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|
| Relations |
| BioProject |
PRJNA541284 |
| SRA |
SRP195561 |
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