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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 17, 2020 |
| Title |
Distinct mesenchymal cell populations generate the essential intestinal BMP signaling gradient |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The intestinal epithelium requires trophic factors and signaling gradients to maintain proper positioning of Intestinal Stem Cells (ISC) and proper differentiation of ISCs. Because the underlying mesenchyme is composed of numerous cell types, we utilized both transgenic and antibody methods to isolate specific mesenchymal populations including GFP-hi and GFP-lo cells from the murine transgenic PDGFRAH2BEGFP mouse (Jackson, #007669), endothelial and lymphatic endothelial cells via antibody for CD31+LYVE1- and CD31+LYVE1+, respectively. We found distinct molecular signatures of these cell types and focused our analyses on known trophic factors of the overlaying intestinal epithelium.
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| Overall design |
Examination of mRNA profiles of distinct mesenchymal cell types.
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| Contributor(s) |
McCarthy N, Manieri E, Storm EE, Saadatpour A, Luoma A, Kapoor VN, Madha S, Gaynor L, Cox C, Keerthivasan S, Wucherpfennig K, Yuan G, de Sauvage FJ, Turley SJ, Shivdasani RA |
| Citation(s) |
32084389, 38042929 |
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| Submission date |
May 03, 2019 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Ramesh Shivdasani |
| E-mail(s) |
ramesh_shivdasani@dfci.harvard.edu
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| Organization name |
Dana Farber Cancer Institute
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| Department |
Medical Oncology
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| Lab |
Shivdasani
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| Street address |
450 Brookline Ave. Dana 720D
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA540975 |
| SRA |
SRP194909 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE130681_RAW.tar |
316.0 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
| GSE130681_Rawgenescounts.csv.gz |
345.4 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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