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| Status |
Public on Jan 22, 2020 |
| Title |
Single-cell RNAseq analysis of testicular germ and somatic cell development during the perinatal period |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pro-spermatogonia (SG) serve as the gateway to spermatogenesis. Using single-cell RNA sequencing (RNAseq), we studied the development of ProSG, their SG descendants, and testicular somatic cells, during the perinatal period in mice. We identified both gene and protein markers for 3 temporally distinct ProSG cell subsets, including a migratory cell population with a distinct transcriptome from the previously defined T1- and T2-ProSG stages. This intermediate (I)-ProSG subset translocates from the center of seminiferous tubules to the spermatogonial stem cell (SSC) “niche” in its periphery soon after birth. We identified 3 undifferentiated SG subsets at postnatal day 7, each of which express distinct genes, including transcription factor and signaling genes. Two of these subsets have the characteristics of newly emergent SSCs. We also molecularly defined the development of Sertoli, Leydig, and peritubular myoid cells during the perinatal period, allowing us to identify candidate signaling pathways acting between somatic and germ cells in a stage-specific manner during the perinatal period. Our study provides a rich resource for those investigating testicular germ and somatic cell developmental during the perinatal period.
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| Overall design |
Single cell sequencing (10X genomics) from unfractionated perinatal testicular cells (embryonic day [E] 18.5, postnatal day [P] 2, and P7; with two biological replicates for each timepoint) were performed for this study.
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| Contributor(s) |
Tan K, Song H, Wilkinson MF |
| Citation(s) |
31964773 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM119128 |
Spermatogonial Stem Cell Establishment |
THE REGENTS OF THE UNIV. OF CALIF., UNIV. OF CALIF., SAN DIEGO |
MILES Frome WILKINSON |
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| |
| Submission date |
May 01, 2019 |
| Last update date |
Jan 25, 2020 |
| Contact name |
Kun Tan |
| E-mail(s) |
kutan@health.ucsd.edu
|
| Organization name |
UCSD
|
| Department |
Department of Obstetrics, Gynecology, and Reproductive Sciences
|
| Lab |
Miles F. Wilkinson
|
| Street address |
2880 Torrey Pines Scenic Dr. Room 4812
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA540697 |
| SRA |
SRP194420 |
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