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| Status |
Public on Jul 15, 2019 |
| Title |
The Developmental Heterogeneity of Human Natural Killer Cells Defined by Single-cell Transcriptome |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The heterogeneity of human NK cells has not been fully-defined. Using single-cell RNA-sequencing technology, we find more human NK subsets than previously defined by cell surface markers. The transcriptome-based pseudotime analysis support that CD56bright NK cells are precursors of CD56dim NK cells with identification of a transitional stage. Our data significantly expand our understanding of the heterogeneity and development of human NK cells.
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| Overall design |
Single-cell RNA-sequencing analysis of NK cells from bone marrow of six healthy donors, blood of two healthy donors and a blood sample from a donor with GATA2T354M mutation
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| Contributor(s) |
Yang C, Siebert JR, Burns R, Gerbec ZJ, Bonacci B, Rymaszewski A, Rau M, Riese MJ, Rao S, Carlson K, Routes JM, Verbsky JW, Thakar MS, Malarkannan S |
| Citation(s) |
31477722 |
| |
| Submission date |
Apr 29, 2019 |
| Last update date |
Sep 11, 2019 |
| Contact name |
Subramaniam Malarkannan |
| E-mail(s) |
malarkannanlab@gmail.com
|
| Organization name |
Versiti Blood Research Institute
|
| Street address |
8727 W Watertown Plank Rd
|
| City |
Milwaukee |
| State/province |
WI |
| ZIP/Postal code |
53213 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA540246 |
| SRA |
SRP194123 |
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