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Series GSE130424 Query DataSets for GSE130424
Status Public on Jul 01, 2019
Title Two distinct subsets are identified from the peritoneal myeloid mononuclear cells expressing both CD11c and CD115
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The peritoneal cavity is home to various immune cells. Previous studies have investigated the heterogenous nature of peritoneal myeloid mononuclear cells. However, up to this date, there are no clear criteria to distinguish peritoneal macrophages and dendritic cells (DCs). In the present study, we delineate the subsets of myeloid mononuclear cells in the mouse peritoneal cavity. Considering phenotypical, functional, and ontogenic features, peritoneal myeloid mononuclear cells are divided into 5 subsets: large peritoneal macrophages (LPMs), small peritoneal macrophages (SPMs), DCs, and 2 MHCII+CD11c+CD115+ subpopulations (i.e., MHCII+CD11c+CD115+CD14-CD206- and MHCII+CD11c+CD115+CD14+CD206+). Among them, 2 subsets of competent antigen presenting cells are demonstrated with distinct functional characteristics, one being DCs and the other being MHCII+CD11c+CD115+CD14-CD206- cells. DCs are able to promote fully activated T cells and superior in expanding cytokine producing inflammatory T cells, whereas MHCII+CD11c+CD115+CD14-CD206- cells generate partially activated T cells and possess a greater ability to induce regulatory T cells under TGF-β and retinoic acid conditions. While the development of DCs and MHCII+CD11c+CD115+CD14-CD206- cells are responsive to the treatment of FLT3L and GM-CSF, the numbers of LPMs, SPMs, and MHCII+CD11c+CD115+CD14+CD206+ cells are only influenced by the injection of GM-CSF. In addition, the analysis of transcriptomes reveals that the gene expression profile of MHCII+CD11c+CD115+CD14+CD206+ cells share high similarity with that of SPMs. Collectively, our study identifies 2 distinct subpopulations of MHCII+CD11c+CD115+ cells, (i) MHCII+CD11c+CD115+CD14-CD206- cells closely related to DCs and (ii) MHCII+CD11c+CD115+CD14+CD206+ cells to SPMs.
Overall design Global mRNA sequencing of MHCII+ peritoneal myeloid mononuclear cells
Contributor(s) Park CG, Sohn M
Citation(s) 31281712, 34079542
Submission date Apr 29, 2019
Last update date Jun 09, 2021
Contact name Chae Gyu Park
Organization name Yonsei University College of Medicine
Department Severance Biomedical Science Institute
Lab Laboratory of Immunology
Street address 50-1 Yonsei-ro
City Seoul
State/province Seoul
ZIP/Postal code 03722
Country South Korea
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (9)
GSM3738428 SPM rep1
GSM3738429 SPM rep2
GSM3738430 P-IV.II rep1
BioProject PRJNA540232
SRA SRP194115

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Supplementary file Size Download File type/resource
GSE130424_Normalized_FPKM.xlsx 2.8 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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