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| Status |
Public on Apr 23, 2020 |
| Title |
Transcriptional Idenetity of Itch-activated Central Amygdala Neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed RNA-seq to identify transcriptional profiles of itch-activated CeA neurons. To do this, we crossed FosTRAP mice to the Cre-dependent tdTomato flox-stop reporter line as described above. We then injected chloroquine into the nape of the neck, paired with injection of 4-OHT to FosTRAP expression of tdTomato in itch-activated neurons. The CeA was harvested, the cells dissociated and sorted by FACS, and tdTomato+ cells were compared to TdTomato- cells.
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| |
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| Overall design |
Facs CeA TdTomato+ve and tdTomato _ve neurons after TRAPping these neurons to chloroquine stimuli.
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| |
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| Contributor(s) |
Samineni V |
| Citation(s) |
34032210 |
| |
| Submission date |
Apr 24, 2019 |
| Last update date |
Jun 08, 2021 |
| Contact name |
Vijay Samineni |
| E-mail(s) |
vksamineni@wustl.edu
|
| Phone |
6183038445
|
| Organization name |
Washington university St. louis
|
| Street address |
4940 Parkview Place, Room 5539 Department of Anesthesiology
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (14)
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| Relations |
| BioProject |
PRJNA534481 |
| SRA |
SRP193753 |
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