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Series GSE130060 Query DataSets for GSE130060
Status Public on Apr 19, 2019
Title Induction and maintenance of specific multipotent progenitor stem cells synergistically mediated by Activin A and BMP4 signaling
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We recently reported that epiblast stem cells (EpiSCs)-like cells could be derived from preimplantation embryos (named as AFSCs). Here, we established AFSCs from preā€implantation embryos of multiple mouse strains and showed that unlike EpiSCs, the derivation efficiency of AFSCs was affected by the genetic background. We then used AFSCs lines to dissect the roles of Activin A (Act A) and basic fibroblast growth factor and reported that Act A alone was capable of maintaining self renewal but not developmental potential in vivo. Finally, we established a novel experimental system, in which AFSCs were efficiently converted to multipotent progenitor stem cells using Act A and BMP4 (named as ABSCs). Importantly, these ABSCs contributed to neural mesodermal progenitors and lateral plate mesoderm in postimplantation chimeras. Taken together, our study established a robust experimental system for
the generation of specific multipotent progenitor stem cells that was self-renewable and capable of contributing to embryonic and extra-embryonic tissues.
Overall design mRNA profiles of 3 cell types, generated by RNA sequencing in 3 duplicates, using HiSeq x10. Paired-end 150-bp sequencing was applied
Contributor(s) Chen Y, Bao S
Citation(s) 32324269
BioProject PRJNA532991
Submission date Apr 18, 2019
Last update date May 21, 2020
Contact name Chen Yanglin
Organization name Southern Medical University
Street address shatai road
City Guangzhou
ZIP/Postal code 510515
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (9)
GSM3731179 AFSCs rep1
GSM3731180 AFSCs rep2
GSM3731181 AFSCs rep3
SRA SRP192724

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Supplementary file Size Download File type/resource
GSE130060_gene_count.txt.gz 2.3 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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