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Series GSE129881 Query DataSets for GSE129881
Status Public on Oct 14, 2019
Title IL-27 receptor signaling regulated stress hematopoiesis drives Abdominal Aortic Aneurysm development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Abdominal Aortic Aneurysm (AAA) is a prevalent life-threatening disease, where aortic wall degradation is mediated by accumulated immune cells. Though cytokines regulate inflammation within the aorta, their contribution to AAA via distant alterations, particularly in the control of hematopoietic stem cell (HSC) differentiation remains poorly defined. Here we report a pathogenic role for the interleukin-27 receptor (IL-27R) in AAA as genetic ablation of IL-27R protects mice from the disease development, where the mitigation of AAA is associated with a blunted accumulation of myeloid cells in the aorta due to the attenuation of Ang II-induced HSC expansion. The loss of IL-27R engages transcriptional programs that promote HSCs quiescence and suppresses differentiation decreasing mature myeloid cell production and accumulation in the aorta. Our studies illuminate how a prominent vascular disease can be distantly driven by cytokine dependent regulation of bone marrow precursors.
 
Overall design RNA-seq for long-term progenitors (LT-HSC), short-term progenitors (ST-HSC) from in Apoe-/-, Apoe-/-Il27ra+/- and Apoe-/-Il27ra-/- mice treated with PBS or AngII
 
Contributor(s) Koltsova EK, Peshkova IO
Citation(s) 31695038
Submission date Apr 16, 2019
Last update date Nov 19, 2019
Contact name Priyankara J Wickramasinghe
E-mail(s) priyaw@wistar.org
Phone 2154956837
Organization name The Wistar Institute
Department Bioinformatics
Lab Genomics
Street address 3601 Spruce Street
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (36)
GSM3723907 LT-HSC cells from Il27ra WT mice treated with PBS, rep 1
GSM3723910 LT-HSC cells from Il27ra +/- mice treated with PBS, rep 1
GSM3723912 LT-HSC cells from Il27ra +/- mice treated with PBS, rep 2
Relations
BioProject PRJNA533084
SRA SRP192713

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Supplementary file Size Download File type/resource
GSE129881_Koltsova_Il27r_RNAseq.txt.gz 1010.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record
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