|
| Status |
Public on Apr 17, 2019 |
| Title |
REPROGRAMMING IDENTIFIES FUNCTIONALLY DISTINCT STAGES OF CLONAL EVOLUTION IN MYELODYSPLASTIC SYNDROMES |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
As part of this study, we isolated induced pluripotent stem cells (iPSCs) from a patient with TP53-mutant MDS and identified loss of chromosome 5q as a cooperating genetic event. Using RNA sequencing we found that loss of chromosome 5q dysregulates genes that maintain chromosome stability, predisposing TP53-mutant cells to chromosomal rearrangements and progression to complex karyotype.
|
| |
|
| Overall design |
Hematopoietic progenitors were derived from induced pluripotent stem cells heterozygous for the TP53 R209fs mutation (TP53+/-) with or without the deletion on chromosome 5q (TP53+/-;del5q). Gene expression was compared between TP53+/- and TP53+/-;del5q HPCs by RNAseq to identify genes significantly downregulated as a result of 5q loss.
|
| |
|
| Contributor(s) |
Hsu J, Reilly A, Hayes BJ, Clough CA, Konnick EQ, Torok-Storb B, Gulsuner S, Wu D, Becker PS, Keel SB, Abkowitz JL, Doulatov S |
| Citation(s) |
31010849 |
| |
| Submission date |
Apr 16, 2019 |
| Last update date |
May 13, 2021 |
| Contact name |
Sergei Doulatov |
| E-mail(s) |
doulatov@uw.edu
|
| Organization name |
University of Washington
|
| Street address |
1795 NE Pacific Street
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98126 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA533068 |
| SRA |
SRP193143 |
Less...
More...